/

/

6-羟基多巴胺诱导的基于斑马鱼的成人帕金森病模型的运动评估

Journal JoVE
Neurosciences

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Neurosciences

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson’s Disease Model

Please note that all translations are automatically generated. Click here for the English version.

6-羟基多巴胺诱导的基于斑马鱼的成人帕金森病模型的运动评估

DOI:

10.3791/63355-v

•

07:32 min

•

December 28, 2021

•

Naemah Md Hamzah, Siong Meng Lim, Yuganthini Vijayanathan², Fei Tieng Lim, Abu Bakar Abdul Majeed, Maw Pin Tan, Kalavathy Ramasamy

¹Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy,Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, ²Department of Medicine, Faculty of Medicine,Universiti Malaya

Chapitres

00:05Introduction
01:00Zebrafish Maintenance and Pre-ICV Microinjection Preparations
02:19Anaesthetisation and ICV Injection of Zebrafish
04:10Locomotor Assessment
05:22Results: Assessment of Neuroregeneration in Zebrafish Based PD Model
06:44Conclusion

Summary

Traduction automatique

本方案描述了在腹侧间脑（Dn）处用神经毒性6-羟基多巴胺（6-OHDA）向成年斑马鱼注射脑室（ICV）以及通过使用开放水箱测试评估损伤和随后恢复的游泳行为，并伴有使用视频跟踪软件进行分析。

Tags

Keywords: Zebrafish Parkinson’s Disease 6-hydroxydopamine Locomotor Assessment Motor Impairment Dopaminergic Neurons Intracerebroventricular Injection Neuroregeneration Cell Replacement Tricaine Methanesulfonate Anesthesia Microcapillary Injector

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

方法来描述自发和惊吓引起的步态中的鱼藤酮诱导的帕金森病模型<em>果蝇</em

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease

胸脊髓截断手术和大鼠开放场运动评估

描述眼动参数与无视力帕金森病患者眼动功能的关系

将RatWalker系统应用于帕金森病遗传大鼠模型中的步态分析

帕金森病的6-羟基多巴胺大鼠模型

左旋多巴诱导的帕金森病大鼠模型中运动障碍的诱导与评估

成年斑马鱼的游泳耐力和游泳行为评估

分析编码人类α突触核蛋白的腺相关病毒载体诱导的帕金森病小鼠模型

使用MouseWalker量化脊髓损伤小鼠模型中的运动功能障碍

Read Article