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Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
DOI:

08:01 min

April 21, 2022

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Chapitres

  • 00:05Introduction
  • 00:45Digestion of Tumor Tissue
  • 03:16Generation of Primary Tumor Cell Line
  • 04:47Characterization and Banking of Established Primary Tumor Cell Line
  • 06:13Results: Determination of Fibroblast Contamination of Cell Culture and Flow Cytometry Surface Staining of Cell Lines
  • 07:12Conclusion

Summary

Traduction automatique

The goal of this method paper is to demonstrate a robust and reproducible methodology for the enrichment, generation, and expansion of primary tumor cell lines from surgically resected pleural mesothelioma.

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