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Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes
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Journal JoVE Biologie
Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes

Quantitating Iron Transport Across the Mouse Placenta In Vivo Using Nonradioactive Iron Isotopes

DOI:
10.3791/63378-v

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08:45 min

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May 10, 2022

•

,
1Center for Iron Disorders, David Geffen School of Medicine,University of California, Los Angeles

Chapitres

  • 00:04Introduction
  • 00:37Preparation of 58Fe‐Transferrin
  • 03:37Administration of <sup58Fe‐Transferrin Intravenously to E17.5 Pregnant Mice
  • 05:53Tissue Processing for Quantitative Iron Analysis by Inductively Coupled Plasma Mass Spectrometry (ICP‐MS)
  • 07:14Results: Physiological Regulation of Mouse Placental Ferroportin During Maternal Iron Deficiency
  • 08:08Conclusion

Summary

Traduction automatique

Traduction automatique

This article demonstrates how to prepare and administer transferrin-bound nonradioactive isotopic iron for studies of iron transport in mouse pregnancy. The approach for quantifying isotopic iron in fetoplacental compartments is also described.

Tags

Iron TransportMouse PlacentaNonradioactive Iron IsotopesStable Iron IsotopesIron UptakeIron RegulationIron DistributionFerric ChlorideFerric NitrilotriacetateApotransferrinTransferrin LoadingMolecular Weight Cutoff Column

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