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一种重力喂养的经心灌注方法，用于小鼠中枢神经系统的组织学分析

Journal JoVE
Neurosciences

This content is Free Access.

Journal JoVE Neurosciences

A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

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一种重力喂养的经心灌注方法，用于小鼠中枢神经系统的组织学分析

DOI:

10.3791/63386-v

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10:37 min

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January 21, 2022

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Arnav Rana, Paul T. Massa, Xin Jie Chen³

¹Department of Biochemistry and Molecular Biology,State University of New York Upstate Medical University, ²Department of Neurology,State University of New York Upstate Medical University, ³Department of Neuroscience and Physiology,State University of New York Upstate Medical University

Chapitres

00:04Introduction
00:46Construction of Perfusion Apparatus and Dissection Platform
02:40Preparation of the Paraformaldehyde Solution
04:58Transcardial “Pump-Free” Perfusion
09:18Results: Histological Analysis Following Gravity-Fed Perfusion
10:09Conclusion

Summary

Traduction automatique

提出了一种用于小鼠中枢神经系统组织学分析的方便的重力喂养灌注方法。磷酸化α突触核蛋白的免疫荧光检测在帕金森病的小鼠模型中得到证明。这项工作还全面描述了经心肌灌注，解剖，组织冷冻/包埋和冷冻切片步骤。

Tags

Keywords: Gravity-fed Transcardial Perfusion Histologic Analysis Mouse Central Nervous System Fixative Perfusion Protocol Apparatus Buffer Bottles Micro Spatulas Tubing Syringe PBS PFA Styrofoam Block

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