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Biochemistry

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JoVE Journal Biochemistry
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
 

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Article DOI: 10.3791/63425-v • 10:46 min • February 2nd, 2022
February 2nd, 2022 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 2, 1
1Department of Chemistry, Ben-Gurion University of the Negev, Israel, 2Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Israel

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The bi-directional mitotic kinesin-5 Cin8 accumulates in clusters that split and merge during their motility. Accumulation in clusters also changes the velocity and directionality of Cin8. Here, a protocol for motility assays with purified Cin8-GFP and analysis of motile properties of single molecules and clusters of Cin8 is described.

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Keywords: Kinesin-5 Cin8 Bidirectional Motor Protein Single-molecule Motility Assay Saccharomyces Cerevisiae GFP-type Full-length Cin8 Protein Purification Nuclear Proteins Fluorescent Particles
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