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JoVE Journal Genetics
Reusable Single Cell for Iterative Epigenomic Analyses
 

Reusable Single Cell for Iterative Epigenomic Analyses

Article DOI: 10.3791/63456-v • 10:28 min • February 11th, 2022
February 11th, 2022 •
1, 2, 3,4, 1
1Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 2Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 3CCR Collaborative Bioinformatics Resource (CCBR), Center for Cancer Research, National Cancer Institute, National Institutes of Health, 4Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute

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résumé
Automatic Translation

The present protocol describes a single-cell method for iterative epigenomic analyses using a reusable single cell. The reusable single cell allows analyses of multiple epigenetic marks in the same single cell and statistical validation of the results.

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Keywords: Reusable Single Cell Epigenomic Analysis Single-cell Epigenome Epigenetic Therapy Epigenome Regulation Gene Expression Intercalator Dye EDTA PBS PCR Tube Swing Rotor Liquid Handling Robot TEMED Mineral Oil TP Magnesium Negative Buffer Annealing Buffer
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