Journal
/
Neurosciences
/
Expanding the Toolkit for In Vivo Imaging of Axonal Transport
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Expanding the Toolkit for In Vivo Imaging of Axonal Transport
Please note that all translations are automatically generated. Click here for the English version.

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

DOI:
10.3791/63471-v

•

09:24 min

•

December 23, 2021

•

, , ,
1Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology,University College London, 2UCL Queen Square Motor Neuron Disease Centre,University College London, 3UK Dementia Research Institute,University College London

Chapitres

  • 00:05Introduction
  • 00:58Presurgery Preparation and Intramuscular Injections
  • 03:16In Vivo Axonal Transport
  • 04:53In Vivo Axonal Imaging
  • 06:55Results: Assessment of In Vivo Axonal Transport of Signaling Endosomes and Mitochondria
  • 08:46Conclusion

Summary

Traduction automatique

Traduction automatique

Using transgenic fluorescent mice, detailed protocols are described to assess in vivo axonal transport of signaling endosomes and mitochondria within motor and sensory axons of the intact sciatic nerve in live animals.

Tags

In Vivo ImagingAxonal TransportSignaling EndosomesMitochondriaPeripheral NeuronsMotor Disease ModelsAgingGlass MicropipetteIntramuscular InjectionsTibialis Anterior MuscleTetanus NeurotoxinSciatic Nerve

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

神经调节和线粒体运输：在较长的时间里，海马神经元的实时成像

使用超声波血脑屏障破坏和锰增强MRI的脑功能成像

初级量子神经元轴突运输的实时成像点标记的BDNF

嗅觉系统作为模型来研究轴突生长的模式和形态<em>在体内</em

在体内轴突兴奋性试验对大鼠尺神经的电生理测量

体内新生小鼠皮层神经元的双光子成像

使用荧光宏镜检查,通过"完整小鼠头骨"进行脑脊液传输的 Vivo 成像

使用微流体腔系统在运动神经元培养物中有机细胞的斧头迁移

切除小鼠神经神经纤维神经丝的成像与分析

小鼠视网膜的经瞳双光子体内成像

Read Article