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High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
 

High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry

Article DOI: 10.3791/63485-v • 10:08 min • July 6th, 2022
July 6th, 2022 •
1,2, 1,2, 1,2, 1, 1,3, 1,2
1Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 2Department of Plant Biology, Morrill Hall, University of Illinois at Urbana-Champaign, 3Environmental Plant Physiology group, Department of Plant Sciences, University of Cambridge

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The protocol introduces a high-throughput method for measuring the relaxation of non-photochemical quenching by pulse amplitude modulated chlorophyll fluorometry. The method is applied to field-grown Glycine max and can be adapted to other species to screen for genetic diversity or breeding populations.

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High-Throughput Analysis Non-Photochemical Quenching Pulse Amplitude Modulated Chlorophyll Fluorometry Genetic Variation Breeding Populations Large Number Of Genotypes Glycine Max Soybean Lift Discs Protocol Handling Lift Disc Water Saturation 24-well Plate Rubber Stopper Humboldt Cork Borer Leaf Discs Technical Replicates
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