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Eksozom Tabanlı Dopamin Taşıyıcı Sistemin Formüle Edilmesi ve Karakterize Edilmesi
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1Vocational School of Health Services/İstanbul,Altinbas University, 2Chemical Metallurgy Faculty, Department of Bioengineering,Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology,Istinye University, 43D Bioprinting Design & Prototyping R&D Center,Istinye University, 5Stem Cell and Tissue Engineering R&D Center,Istinye University, 6Medicine Faculty,Istinye University, 7Vocational School of Health Services,Istinye University, 8The V. Akhundov National Scientific Research Medical Prophylactic Institute, 9Center for Stem Cells and Regenerative Medicine (LivMedCell),Liv Hospital
Chapitres
- 00:04Introduction
- 00:56Culturing and Cryopreservation of Wharton’s Jelly Mesenchymal Stem Cells
- 01:30Production of Exosomes from Wharton’s Jelly Mesenchymal Stem Cells
- 02:20Dopamine Loading into Exosomes and Characterization of Dopamine Loaded Exosomes
- 03:15Results: Development of a Nanocarrier System by Encapsulation of Dopamine into Exosomes Isolated from WJ‐MSCs
- 05:07Conclusion
Bu çalışmada, Wharton'un jöle mezenkimal kök hücrelerinden izole edilen kök hücrelerin eksozomlarının dopamin yüklemesi ile bir formülasyon elde etmeyi amaçladık. Eksozom izolasyonu ve karakterizasyonu, elde edilen eksozomlara ilaç yüklemesi ve geliştirilen formülasyonun sitotoksik aktivitesi bu protokolde tanımlanmıştır.
