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Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids
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Journal JoVE Biologie
Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

DOI:
10.3791/63684-v

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10:12 min

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March 23, 2022

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, , , , , , ,
1Department of Microbiology, Li Ka Shing Faculty of Medicine,The University of Hong Kong, 2Centre for Virology, Vaccinology and Therapeutics,Hong Kong Science and Technology Park, 3State Key Laboratory of Emerging Infectious Diseases,The University of Hong Kong

Chapitres

  • 00:04Introuduction
  • 01:16Derivation of Human Lung Organoid
  • 03:25Expansion of Human Lung Organoids
  • 05:31Proximal Differentiation to Generate Mature Airway Organoids
  • 07:42Results: Deriving Human Lung Organoids and Proximal Differentiation to Generate 3D and 2D Airway Organoids
  • 09:38Conclusion

Summary

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Traduction automatique

The protocol presents a method to derive human lung organoids from primary lung tissues, expand the lung organoids and induce proximal differentiation to generate 3D and 2D airway organoids that faithfully phenocopy the human airway epithelium.

Tags

Lung OrganoidsAirway Epithelium3D CultureCell IsolationCollagenase DigestionBasement MatrixExpansion MediumProximal Differentiation

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