Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Introduction
- 01:24Seeding of Organoid Fragments
- 03:14Dynamic Culture of Intestine Chip
- 04:19CYP450 Induction in the Intestine Chip
- 05:49Sample Preparation for LC-MS Analysis
- 07:15Stimulation with IFNγ
- 08:24Results: CYP3A4 Induction and IFNγ Stimulation in the Intestine Chip
- 10:12Conclusion
Tags
Human OrganoidsOrgan-on-a-chipIntestinal Region-specific FunctionalityIntestinal EpitheliumPharmacokineticsDrug-drug InteractionIntestinal Epithelial Barrier DysfunctionIntestinal PhysiologyPharmacodynamicsSeedingOrganoid FragmentsBMM DissociationOrganoid DigestionAdvanced DMEM F12Organoid Growth MediumCoated Chips
