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Electroporation-Mediated Delivery of Cas9 Ribonucleoproteins and mRNA into Freshly Isolated Primary Mouse Hepatocytes
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Chapitres
- 00:04Introduction
- 01:18Animal Surgery
- 03:26Hepatocyte Isolation
- 04:54Electroporation and Cell Culture
- 07:46Results: Generating Electroporation-Assisted CRISPR-Cas9 Edits in Freshly Isolated Mouse Hepatocytes
- 09:06Conclusion
This protocol describes techniques for isolating primary mouse hepatocytes from the liver and electroporating CRISPR-Cas9 as ribonucleoproteins and mRNA to disrupt a therapeutic target gene associated with an inherited metabolic disease of the liver. The methods described result in high viability and high levels of gene modification after electroporation.
