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The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
Journal JoVE
Biologie
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Journal JoVE Biologie
The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
DOI:

06:34 min

April 28, 2022

Chapitres

  • 00:04Introduction
  • 00:44Caco-2 Cell Culture on Multiwell Plates
  • 02:03Preparation of Insert Rings
  • 02:53Preparation of Pancreatin-Bile Solution
  • 03:20In Vitro Digestion Initiation
  • 05:19Results: Measurement and Evaluation of Iron Bioavailability
  • 05:48Conclusion

Summary

Traduction automatique

The Caco-2 cell bioassay for iron (Fe) bioavailability represents a cost-effective and versatile approach to assess Fe bioavailability from foods, food products, supplements, meals, and even diet regimens. Thoroughly validated to human studies, it represents the state of the art for studies of Fe bioavailability.

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