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JoVE Journal Neuroscience
Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle
 

Visualizing the Morphological Characteristics of Neuromuscular Junction in Rat Medial Gastrocnemius Muscle

Article DOI: 10.3791/63954-v • 08:42 min • May 17th, 2022
May 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

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The protocol shows a method to examine spatial correlation among the pre-synaptic terminals, post-synaptic receptors, and peri-synaptic Schwann cells in the rat medial gastrocnemius muscle using fluorescent immunohistochemistry with different biomarkers, namely, neurofilament 200, vesicular acetylcholine transporter, alpha-bungarotoxin, and S100.

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Neuromuscular Junction Rat Medial Gastrocnemius Muscle Morphological Characteristics Integrity Plasticity Schwann Cells Pre And Post Lab Animals Euthanizing Thoracic Cavity Intravenous Catheter Perfusion Normal Saline Paraformaldehyde Phosphate Buffer Hind Limb Sciatic Nerve Cryoprotect Sucrose Solution Freezing Stage Sliding Microtome System
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