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成年小鼠大脑中诱导荧光标记干细胞的谱系示踪

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Neurosciences

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Journal JoVE Neurosciences

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

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成年小鼠大脑中诱导荧光标记干细胞的谱系示踪

DOI:

10.3791/63998-v

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09:44 min

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May 20, 2022

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Gabriel S. Jensen², Jake W. Willows, David T. Breault, Kristy L. Townsend²

¹Graduate School of Biomedical Science and Engineering,University of Maine, ²Department of Neurosurgery,Ohio State University Wexner Medical Center, ³Boston Children’s Hospital,Harvard Medical School

Chapitres

00:04Introduction
01:19Standard Immunostaining Procedure of Thinly Sliced Brain Sections on Slides
03:36Alternate Processing of Lineage-Traced Brains by iDISCO Brain Clearing and Whole Mount Imaging
06:53Imaging of Stained Slides or Cleared Brains
07:27Results: Lineage Tracing of Adult mTert-rtTA::oTet-Cre::Rosa-mTmG Mouse Brains
09:04Conclusion

Summary

Traduction automatique

使用诱导的转基因谱系追踪小鼠系，用荧光团永久标记干细胞及其后代的能力允许对体内的活化，增殖，迁移和/或分化进行空间和时间分析。谱系追踪可以揭示有关谱系承诺、对干预的反应和多能性的新信息。

Tags

Keywords: Lineage Tracing Inducible Fluorescent Labeling Adult Mouse Brain Stem Cells Transgenic Immunostaining Immunofluorescence Antigen Retrieval Triton X-100 Tween-20 Tyrogen Black Alexa Fluor GFP Brain Plasticity Neurodegenerative Diseases Aging

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