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Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes
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Médecine
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Journal JoVE Médecine
Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes

Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes

DOI:
10.3791/64055-v

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07:32 min

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May 25, 2022

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,
1Department of Cardiac Surgery, Michigan Medicine,University of Michigan, Ann Arbor

Chapitres

  • 00:05Introduction
  • 00:40Contractile Function Analysis of Adult Rat Cardiac Myocytes
  • 04:20Data Analysis of Contractile Function in Isolated Myocytes
  • 06:18Results: Contractile Function in Cardiac Myocytes in Response to Pressure Overload (PO) and Gene Transfer
  • 06:59Conclusion

Summary

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Traduction automatique

A set of protocols are presented that describe the measurement of contractile function via sarcomere length detection along with calcium (Ca2+) transient measurement in isolated rat myocytes. The application of this approach for studies in animal models of heart failure is also included.

Tags

Cardiac Contractile DysfunctionCa2+ TransientsRodent MyocytesHeart FailureContractile FunctionNeurohormonesTherapeutic AgentsSarcomere Length ImagingContractile Function PlatformPeristaltic PumpCell StimulatorMedia PerfusionCover Slip ChamberMicroscopeHeating SystemPacing Stimulator

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