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1Key Laboratory of Sustainable Forest Ecosystem Management – Ministry of Education,Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences
Chapitres
- 00:05Introduction
- 00:33Target Design and In Vitro Synthesis of sgRNA
- 01:18Embryo Collection and Preparation
- 02:39Microinjection of Embryos
- 04:24Post-Injection Insect Rearing
- 05:07Mutant Screening
- 06:41Results: Mutant Screening in B. dorsalis
- 07:39Conclusion
