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Biochemistry

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JoVE Journal Biochemistry
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
 

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Article DOI: 10.3791/64200-v • 09:49 min • November 18th, 2022
November 18th, 2022 •
1, 2, 2, 2,3, 1
1Department of Chemistry, University of Florida, 2Division of Protective Immunity, Children’s Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania

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résumé
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A novel sample preparation method is demonstrated for the analysis of agar-based, bacterial macrocolonies via matrix-assisted laser desorption/ionization imaging mass spectrometry.

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Microbial Cooperation Imaging Mass Spectrometry Bacterial Colonies Agar Tissue Infection Spatial Metabolism Imaging Mass Spectrometry Workflow Bacterial Co-cultures MALDI Matrix Application Metabolites Microbial Pathogens Sample Types Cellular Biochemistry Tissue Biochemistry Dehydration Bacterial Culture Macrocolonies Agarose Bacterial Colonies Microscope Slides Dry Box Dessicant Agar Surface Deformations
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