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Bioengineering

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JoVE Journal Bioengineering
Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants
 

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

Article DOI: 10.3791/64371-v • 08:06 min • October 28th, 2022
October 28th, 2022 •
1, 2, 1,2, 1
1Laboratory of Cell Biology, Department of Orthopaedic Surgery, University Hospital Tübingen, 2Department of Oral and Maxillofacial Surgery, University Hospital Tübingen

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résumé
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We present a step-by-step approach to identify and address the most common problems associated with atomic force microscopy micro-indentations. We exemplify the emerging problems on native human articular cartilage explants characterized by various degrees of osteoarthritis-driven degeneration.

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Keywords: Articular Cartilage Osteoarthritis Micro-indentation Atomic Force Microscopy Cartilage Explants Biomechanical Properties In Vitro Model Cartilage Disc Fluorescence Microscopy AFM Calibration
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