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Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates
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Journal JoVE Médecine
Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

DOI:
10.3791/64424-v

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06:10 min

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August 23, 2022

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, , , , ,
1Department of Pediatric Surgery,University Medical Center Hamburg-Eppendorf, 2Research Laboratory for Pediatric Surgery, 3Research Animal Facility,University Medical Center Hamburg-Eppendorf

Chapitres

  • 00:05Introduction
  • 01:06Access to the Peritoneal Cavity
  • 02:00En-Bloc-Resection
  • 03:19Gall Bladder and Bile Duct Dissection
  • 04:46Results: EBDS Dissection from Murine Neonates
  • 05:23Conclusion

Summary

Traduction automatique

Traduction automatique

For the observation of murine neonatal bile duct disorders, an intact bile duct and efficient preparation are required. Therefore, a new approach for isolating the entire extrahepatic bile duct system in murine neonates was successfully developed while maintaining the integrity of the bile duct.

Tags

Extrahepatic Bile DuctGallbladderDissectionMouse NeonatesBile Duct DisordersDuodenal AtresiaPeritoneumLiverStomachSmall IntestinesColonGallbladderBile Duct SystemDuodenal PapillaDuodenumPyloric AreaEn-bloc Mobilization

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