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Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
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Journal JoVE Biologie
Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

DOI:
10.3791/64584-v

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06:01 min

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October 06, 2022

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, , , , ,
1Renal-Electrolyte Division, Department of Medicine,University of Pittsburgh School of Medicine

Chapitres

  • 00:04Introduction
  • 00:48Transduction of Rodent Bladder
  • 04:08Results: Transducing the Native Rodent Urothelium for Expression of Transgenes or Downregulation of Endogenous Gene Products
  • 05:33Conclusion

Summary

Traduction automatique

Traduction automatique

Methods are described for the generation of large amounts of recombinant adenoviruses, which can then be used to transduce the native rodent urothelium allowing for expression of transgenes or downregulation of endogenous gene products.

Tags

Adenovirus-mediated TransductionBladder UrotheliumCDNASiRNA ExpressionCatheterizationN-dodecyl-beta-D-maltosidePBS WashAnesthetized MouseGene DeliveryUrothelial Gene Expression

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