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Gerando e Imageando Organoides Epitéliares de Camundongos e Humanos a partir de Tecido Mamário Normal e Tumoral Sem Passagem
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1Department of Internal Medicine, Division of Hematology and Oncology,University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center,University of Texas Southwestern Medical Center, 3Department of Molecular Biology,University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine,University of Texas Southwestern Medical Center, 5Department of Cell Biology,University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine,University of Southern California
Chapitres
- 00:04Introduction
- 00:55Collecting, Digesting Tissue, and Differential Centrifugation
- 04:11Embedding Organoids in BECM and Collagen
- 07:22Results: Immunofluorescent Imaging of Embedded Organoids
- 08:11Conclusion
Este protocolo discute uma abordagem para a geração de organoides epiteliais a partir de tecido mamário primário normal e tumoral através da centrifugação diferencial. Além disso, estão incluídas instruções para a cultura tridimensional, bem como imagens imunofluorescentes de organoides incorporados.
