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流式细胞术 检测 接种乙型脑炎疫苗的儿童多功能T细胞
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
DOI:

09:37 min

September 23, 2022

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Chapitres

  • 00:05Introduction
  • 00:39Stimulation of PBMCs by Inactivated JEV Particles to Induce Cytokine Expression and Ex Vivo Intracellular Staining
  • 04:28Flow Cytometry Set-Up, Gating Strategy, and Data Analysis
  • 06:56Results: Flow Cytometry Analysis of Polyfunctional T Cell Profiles in PBMCs Within Japanese Encephalitis Virus Vaccinated Children
  • 09:12Conclusion

Summary

Traduction automatique

本协议结合了 离体 刺激和流式细胞术,以分析日本脑炎病毒(JEV)疫苗接种的儿童中外周血单核细胞(PBMC)中的多功能T细胞(TPF)谱。对JEV特异性TPF的检测方法和流式细胞术配色方案进行了测试,为类似研究提供参考。

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