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体内通过乳腺导管内注射将基因传递到小鼠乳腺上皮细胞

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Recherche en cancérologie

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Journal JoVE Recherche en cancérologie

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

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体内通过乳腺导管内注射将基因传递到小鼠乳腺上皮细胞

DOI:

10.3791/64718-v

•

05:36 min

•

February 10, 2023

•

Wen Bu², Yi Li³

¹Lester & Sue Smith Breast Center,Baylor College of Medicine, ²Department of Medicine,Baylor College of Medicine, ³Department of Molecular & Cellular Biology,Baylor College of Medicine, Houston, TX 77030

Chapitres

00:04Introduction
00:39Syringe and Virus Preparation
01:47Animal Preparation
02:27Intraductal Injection of Virus
03:14Detection of Infected Cells by a Fluorescent Stereomicroscope
03:58Results: Analyzing the Mammary Epithelial Cells After the Intraductal Injection
04:57Conclusion

Summary

Traduction automatique

本协议描述了通过在导管内注射病毒载体以将感兴趣的基因递送到乳腺上皮细胞中。

Tags

In Vivo Gene Delivery Mammary Epithelial Cells Mammary Intraductal Injection Mouse Model Tumor Genesis Viral Transduction Bromophenol Blue Anesthesia Nipple Injection Mammary Gland Dissection

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