Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:04Introduction
- 00:56Cell Viability Assay
- 03:02Screening of Optimal Combination of Two Drugs and its Protective Effect Evaluation
- 03:37Annexin V-FITC/PI Assay for Apoptosis Rate
- 05:00Immunofluorescence Detection of Caspase-3 Generation
- 06:16Immunofluorescence Assay of ROS Level
- 06:50Western Blot Detection of Protein Expression of Nrf2, p-Akt, Akt, Bcl-2, and Bax
- 08:11Results: Effects of Drug Combinations on the Normal and Injured PC12 Cells
- 10:01Conclusion
