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1Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology,Shanghai Jiao Tong University, 2Joint Center for Single Cell Biology, School of Agriculture and Biology,Shanghai Jiao Tong University, 3Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education),Shanghai Jiao Tong University
Chapitres
- 00:05Introduction
- 00:36Plant Culture
- 01:20Tissue Preparation and Pretreatment
- 02:37Setting up the Protocol with the Microplate Reader, Establishing the Elicitation System, and Measuring Real-Time ROS Production
- 03:42Results: ROS Induction, the Total Amount of ROS Generated, and ROS Production in the Exposed Cells of the Cut Edge
- 05:07Conclusion
Burada, patojenle ilişkili moleküler paternle tetiklenen immün yanıtta pirinç dokularında apoplastik reaktif oksijen türlerinin (ROS) üretiminin gerçek zamanlı tespiti için bir yöntem açıklanmaktadır. Bu yöntem basit, standartlaştırılmış ve kontrollü koşullar altında yüksek oranda tekrarlanabilir sonuçlar üretir.
