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AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells

Article DOI: 10.3791/64857-v • 06:03 min • June 23rd, 2023
June 23rd, 2023 •
1, 1,2, 3, 4, 5, 6, 1, 1
1Institute of Mathematics and Statistics, University of São Paulo, 2Center for Mathematics, Computing and Cognition, Federal University of ABC, 3Department of Botany, Institute of Biosciences, University of São Paulo, 4Cell Biology and Molecular Genetics Department, University of Maryland, 5Department of Biosciences, University of Milan, 6Department of Physics, Politecnico di Milano

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Current methods for analyzing the intracellular dynamics of polarized single cells are often manual and lack standardization. This manuscript introduces a novel image analysis pipeline for automating midline extraction of single polarized cells and quantifying spatiotemporal behavior from time lapses in a user-friendly online interface.

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Keywords: AMEBaS Midline Extraction Background Subtraction Ratiometric Fluorescence Time-lapse Polarized Single Cells Automation Cell Segmentation Cell Midline Tracing Skeletonization Linear Extrapolation Interpolation
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