Journal
/
Biologie
/
Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence
Journal JoVE
Biologie
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence

Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence

DOI:
10.3791/65005-v

•

08:07 min

•

April 28, 2023

•

, , , ,
1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL),Universidad de Buenos Aires, CONICET, 2Signalling Programme,The Babraham Institute

Chapitres

  • 00:05Introduction
  • 00:49PANC-1 Cell Preparation and Gemcitabine Treatment
  • 01:56Fixation, Permeabilization, and Blocking the PANC-1 Cells
  • 02:32Antibody Incubation
  • 03:57DAPI Staining, Montage, and Visualization of the Cells
  • 04:51LC3 Dots Quantification
  • 06:13Results: Effect of LC3 Immunofluorescence in Pancreatic Cell Lines
  • 07:26Conclusion

Summary

Traduction automatique

Traduction automatique

The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification.

Tags

AutophagyLC3 ImmunofluorescencePancreatic CellsCell PreparationGemcitabine TreatmentCell Fixation And PermeabilizationPrimary Antibody IncubationFluorescent Imaging

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/65005?article_type=v

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
check_url/fr/65005?article_type=v

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
check_url/fr/65005?article_type=v

Measurement of Heme Synthesis Levels in Mammalian Cells
check_url/fr/65005?article_type=v

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
check_url/fr/65005?article_type=v

Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines
check_url/fr/65005?article_type=v

Activating Autophagy by Aerobic Exercise in Mice
check_url/fr/65005?article_type=v

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
check_url/fr/65005?article_type=v

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
check_url/fr/65005?article_type=v

Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body
check_url/fr/65005?article_type=v

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Read Article