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Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
 

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Article DOI: 10.3791/65053-v 09:26 min June 30th, 2023
June 30th, 2023

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résumé

This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization.

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Keywords: Wastewater-based Epidemiology SARS-CoV-2 COVID-19 Air Sampling Viral RNA RT-qPCR Mengovirus N1 Gene N2 Gene RNA Extraction Centrifugal Ultrafiltration Composite Wastewater Sample Molecular Biology
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