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Immunologie et infection

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富集分枝杆菌的天然和重组细胞外囊泡

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Immunologie et infection

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Journal JoVE Immunologie et infection

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

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富集分枝杆菌的天然和重组细胞外囊泡

DOI:

10.3791/65138-v

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06:38 min

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December 08, 2023

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Praapti Jayaswal, Mohd Ilyas, Kuljit Singh², Saurabh Kumar³, Lovely Sisodiya, Sapna Jain, Rahul Mahlawat, Nishant Sharma⁴, Vishal Gupta, Krishnamohan Atmakuri

¹Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute,NCR Biotech Science Cluster, ²Clinical Microbiology Division,CSIR-Indian Institute of Integrative Medicine, ³ICAR-Research Complex for Eastern Region, ⁴Public Health Research Institute,Rutgers University

Chapitres

00:00Introduction
00:42Growing Mycobacterium smegmatis
02:31Enrichment of Mycobacterium smegmatis EVs by Density Gradient Centrifugation
05:24Results: Verifying Enrichment of MsmEVs Containing the EsxA Protein of M. tuberculosis
06:19Conclusion

Summary

Traduction automatique

该方案详细介绍了从耻垢分枝杆菌（Msm）的无轴培养物中富集天然分枝杆菌细胞外囊泡（mEV），以及如何设计和富集含有mCherry（红色荧光报告基因）的重组MsmEV。最后，通过富集含有结核分枝杆菌EsxA蛋白的MsmEVs验证了新方法。

Tags

Keywords: Extracellular Vesicles Mycobacteria Enrichment Recombinant Vaccine Candidates Affinity-based Approaches Mycobacterium Smegmatis Sautons Media Tween 80 Mid-exponential Stage Centrifugation MCherry Fluorescent Proteins

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