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Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice
Journal JoVE
Biologie
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Journal JoVE Biologie
Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice
DOI:

04:10 min

March 31, 2023

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Chapitres

  • 00:04Introduction
  • 00:29Preparation of Intubation Platform and Test Compound
  • 01:39Non‐Invasive Oropharyngeal Instillation
  • 02:56Results: Effect of LPS Tracheal Instillation on Acute Lung Injury
  • 03:38Conclusion

Summary

Traduction automatique

Here, we propose a protocol for intratracheal lipopolysaccharide (LPS) delivery via noninvasive oropharyngeal endotracheal intubation. This method minimizes the trauma of the surgical procedure for the animal and accurately delivers LPS to the trachea and then to the lungs.

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