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Journal
/
Biologie
/
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
/
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE
Biologie
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
DOI:
10.3791/65611-v
•
01:58 min
•
July 28, 2023
•
Lucas Stalder
,
Fabian Koechl
,
Kerstin Hahn
,
Marc Sultan
,
Megana K. Prasad
1
Roche Pharma Research and Early Development, Pharmaceutical Sciences
,
Roche Innovation Centre Basel
Tags
Single Nuclei Isolation
Single Nucleus Sequencing
Automated Nuclei Extraction
Standardized Protocols
Drug Discovery
Transcriptomics
Gene Expression
Robotic Dissociation
Chemical Gradient Filtration
Automated Cell Counting
Mouse Brain
Rat Kidney
Cynomolgus Liver And Spleen
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A Simple, Quick, and Partially Automated Protocol for the Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nucleus Sequencing
check_url/fr/65611?article_type=v
Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing
check_url/fr/65611?article_type=v
Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues
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