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Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
 
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Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

Article DOI: 10.3791/690-v • 07:54 min • March 28th, 2008
March 28th, 2008 •
1, 1
1Department of Cell and Tissue Biology, University of California, San Francisco - UCSF

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Proteins bind to filamentous actin (F-actin) through distinct actin binding modules. In this video we demonstrate the procedure of actin co-sedimentation, which is an in vitro assay routinely used to analyze proteins or specific domains that bind F-actin.

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Actin Co-sedimentation Assay Protein Binding F-actin Actin Cytoskeleton Cellular Processes Dynamic Structure Remodeling Protein Analysis In Vitro Assay Ultracentrifugation Protein Co-sedimenting Actin Binding Affinities Competition Assays
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