/

/

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

DOI:

•

13:02 min

•

April 07, 2008

•

G. Grant Welstead, Tobias Brambrink, Rudolf Jaenisch

¹Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

Chapitres

00:10Cellular Reprogramming
03:30Introduction
04:32Transfecting HEK293T Cells and Purifying Lentivirus
06:08Infection of MEFs with Lentivirus
08:56Picking up the iPS Colonies
11:15Expansion of iPS Cells
12:32Conclusion

Summary

Traduction automatique

This video shows the procedure for generating induced pluripotent stem cells using inducible lentivirus that express Oct4, Sox2, c-Myc and Klf4.

Tags

IPS Cells MEFS Forced Expression Sox-2 Oct-4 C-Myc Klf4 Pluripotency Viral Transduction Reprogramming Strategy Doxycycline-inducible Lentiviral Vectors Induced Pluripotent Stem Cells Mouse Embryonic Fibroblasts Inducible Lentiviruses Retroviral Infection Gene Expression Analysis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Read Article