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1Department of Cancer Genetics and Developmental Biology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Department of Pathology and Laboratory Medicine,University of British Columbia – UBC, 4Photography/Video Production, Multi-Media Services,BC Cancer Agency, 5Department of Medical Genetics, Life Sciences Institute,,University of British Columbia – UBC
Chapitres
- 00:06Introduction
- 02:06The MeDIP Protocol
- 02:25Materials
- 02:56DNA Preparation
- 04:52DNA Sonication
- 06:33Immunoprecipitation of Methylated DNA
- 13:51DNA Purification
- 19:27PCR Validation
- 21:09Credits
Este vídeo demonstra o protocolo para imunoprecipitação DNA metilado (MEDIP). MEDIP é um procedimento dois dias que, seletivamente, extratos de fragmentos de DNA metilado partir de uma amostra do DNA genômico utilizando anticorpos com especificidade para 5-metilcitosina (anti-5 mC).
Tags
Methylated DNA ImmunoprecipitationDNA Methylation PatternsEpigeneticsGene ExpressionNormal DevelopmentDiseaseMethylation ProfilesMeDIPMethylated DNA ExtractionAntibody ReactionImmunoprecipitated DNAInput DNAHeat DenaturationAnti-5’mC AntibodyMagnetic BeadsSecondary AntibodyAntibody ComplexSeparation Of Methylated DNANon-methylated DNA Removal
