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甲基化的DNA免疫沉淀

Journal JoVE
Biologie

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Journal JoVE Biologie

Methylated DNA Immunoprecipitation

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甲基化的DNA免疫沉淀

DOI:

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21:24 min

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January 02, 2009

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Kelsie L. Thu*^1,2, Emily A. Vucic*^1,3, Jennifer Y. Kennett³, Cameron Heryet, Carolyn J. Brown, Wan L. Lam^2,3, Ian M. Wilson³

¹Department of Cancer Genetics and Developmental Biology,BC Cancer Research Centre, ²Interdisciplinary Oncology Program,University of British Columbia – UBC, ³Department of Pathology and Laboratory Medicine,University of British Columbia – UBC, ⁴Photography/Video Production, Multi-Media Services,BC Cancer Agency, ⁵Department of Medical Genetics, Life Sciences Institute,,University of British Columbia – UBC

Chapitres

00:06Introduction
02:06The MeDIP Protocol
02:25Materials
02:56DNA Preparation
04:52DNA Sonication
06:33Immunoprecipitation of Methylated DNA
13:51DNA Purification
19:27PCR Validation
21:09Credits

Summary

Traduction automatique

这个视频演示的甲基化的DNA免疫（MeDIP）协议。 MeDIP是有选择性地从基因组DNA样本中提取5 - 甲基胞嘧啶甲基化的DNA片段具有特异性抗体（抗- 5 MC）为期两天的过程。

Tags

Methylated DNA Immunoprecipitation DNA Methylation Patterns Epigenetics Gene Expression Normal Development Disease Methylation Profiles MeDIP Methylated DNA Extraction Antibody Reaction Immunoprecipitated DNA Input DNA Heat Denaturation Anti-5’mC Antibody Magnetic Beads Secondary Antibody Antibody Complex Separation Of Methylated DNA Non-methylated DNA Removal

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