Andrew G. Elefanty Murdoch Childrens Research Institute The Royal Childrens Hospital Biography Publications Institution JoVE Articles Andrew G. Elefanty has not added a biography. If you are Andrew G. Elefanty and would like to personalize this page please email our Author Liaison for assistance. Publications Human Definitive Haemogenic Endothelium and Arterial Vascular Endothelium Represent Distinct Lineages Nature Cell Biology. May, 2015 | Pubmed ID: 25915127 Productive Infection of Human Embryonic Stem Cell-Derived NKX2.1+ Respiratory Progenitors With Human Rhinovirus Stem Cells Translational Medicine. Apr, 2015 | Pubmed ID: 25873746 Efficient Generation of NKX6-1(+) Pancreatic Progenitors from Multiple Human Pluripotent Stem Cell Lines Stem Cell Reports. Apr, 2015 | Pubmed ID: 25843049 β-cell Regeneration and Differentiation: How Close Are We to the 'holy Grail'? Journal of Molecular Endocrinology. Dec, 2014 | Pubmed ID: 25385843 Derivation of Insulin-producing Beta-cells from Human Pluripotent Stem Cells The Review of Diabetic Studies : RDS. 2014 | Pubmed ID: 25148364 SIRPA, VCAM1 and CD34 Identify Discrete Lineages During Early Human Cardiovascular Development Stem Cell Research. Jul, 2014 | Pubmed ID: 24968096 FOXN1 (GFP/w) Reporter HESCs Enable Identification of Integrin-β4, HLA-DR, and EpCAM As Markers of Human PSC-derived FOXN1(+) Thymic Epithelial Progenitors Stem Cell Reports. Jun, 2014 | Pubmed ID: 24936476 Efficient Endoderm Induction from Human Pluripotent Stem Cells by Logically Directing Signals Controlling Lineage Bifurcations Cell Stem Cell. Feb, 2014 | Pubmed ID: 24412311 Fourier Transform Infrared Microspectroscopy Reveals Unique Phenotypes for Human Embryonic and Induced Pluripotent Stem Cell Lines and Their Progeny Journal of Biophotonics. Oct, 2014 | Pubmed ID: 23616434 PGC-1α and Reactive Oxygen Species Regulate Human Embryonic Stem Cell-derived Cardiomyocyte Function Stem Cell Reports. 2013 | Pubmed ID: 24371810 The Characterisation of Pluripotent and Multipotent Stem Cells Using Fourier Transform Infrared Microspectroscopy International Journal of Molecular Sciences. 2013 | Pubmed ID: 24065090 WNT3A Promotes Hematopoietic or Mesenchymal Differentiation from HESCs Depending on the Time of Exposure Stem Cell Reports. 2013 | Pubmed ID: 24052942 Fourier Transform Infrared Microspectroscopy Reveals That Tissue Culture Conditions Affect the Macromolecular Phenotype of Human Embryonic Stem Cells The Analyst. Jul, 2013 | Pubmed ID: 23745179 Functional Maturation of HPSC-derived Forebrain Interneurons Requires an Extended Timeline and Mimics Human Neural Development Cell Stem Cell. May, 2013 | Pubmed ID: 23642366 Directed Differentiation and Functional Maturation of Cortical Interneurons from Human Embryonic Stem Cells Cell Stem Cell. May, 2013 | Pubmed ID: 23642365 Generation of Megakaryocytic Progenitors from Human Embryonic Stem Cells in a Feeder- and Serum-free Medium PloS One. 2013 | Pubmed ID: 23424635 Human Embryonic Stem Cell Derived Mesenchymal Progenitors Express Cardiac Markers but Do Not Form Contractile Cardiomyocytes PloS One. 2013 | Pubmed ID: 23342164 Derivation of Endothelial Cells from Human Embryonic Stem Cells in Fully Defined Medium Enables Identification of Lysophosphatidic Acid and Platelet Activating Factor As Regulators of ENOS Localization Stem Cell Research. Jan, 2013 | Pubmed ID: 23164599 Microbioreactor Arrays for Full Factorial Screening of Exogenous and Paracrine Factors in Human Embryonic Stem Cell Differentiation PloS One. 2012 | Pubmed ID: 23300662 Analysis of Mitochondrial Function and Localisation During Human Embryonic Stem Cell Differentiation in Vitro PloS One. 2012 | Pubmed ID: 23284940 Differentiation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells to Cardiomyocytes: a Methods Overview Circulation Research. Jul, 2012 | Pubmed ID: 22821908 APELIN Promotes Hematopoiesis from Human Embryonic Stem Cells Blood. Jun, 2012 | Pubmed ID: 22611158 The Mix Family of Homeobox Genes--key Regulators of Mesendoderm Formation During Vertebrate Development Developmental Biology. Jul, 2012 | Pubmed ID: 22580160 Efficient Generation of Adipocytes in a Dish Nature Cell Biology. Feb, 2012 | Pubmed ID: 22298041 Generating GABAergic Cerebral Cortical Interneurons from Mouse and Human Embryonic Stem Cells Stem Cell Research. May, 2012 | Pubmed ID: 22280980 Pdgfrα and Flk1 Are Direct Target Genes of Mixl1 in Differentiating Embryonic Stem Cells Stem Cell Research. Mar, 2012 | Pubmed ID: 22265737 Temporal Restriction of Pancreatic Branching Competence During Embryogenesis is Mirrored in Differentiating Embryonic Stem Cells Stem Cells and Development. Jul, 2012 | Pubmed ID: 22034992 Brachyury and Related Tbx Proteins Interact with the Mixl1 Homeodomain Protein and Negatively Regulate Mixl1 Transcriptional Activity PloS One. 2011 | Pubmed ID: 22164283 SIRPA is a Specific Cell-surface Marker for Isolating Cardiomyocytes Derived from Human Pluripotent Stem Cells Nature Biotechnology. Nov, 2011 | Pubmed ID: 22020386 NKX2-5(eGFP/w) HESCs for Isolation of Human Cardiac Progenitors and Cardiomyocytes Nature Methods. Dec, 2011 | Pubmed ID: 22020065 Generation of Human Female Reproductive Tract Epithelium from Human Embryonic Stem Cells PloS One. 2011 | Pubmed ID: 21698266 A Targeted NKX2.1 Human Embryonic Stem Cell Reporter Line Enables Identification of Human Basal Forebrain Derivatives Stem Cells (Dayton, Ohio). Mar, 2011 | Pubmed ID: 21425409 Stage-specific Signaling Through TGFβ Family Members and WNT Regulates Patterning and Pancreatic Specification of Human Pluripotent Stem Cells Development (Cambridge, England). Mar, 2011 | Pubmed ID: 21270052 Defined Substrates for Pluripotent Stem Cells: Are We There Yet? Nature Methods. Dec, 2010 | Pubmed ID: 21116246 On the Streets of San Francisco: Highlights from the ISSCR Annual Meeting 2010 Cell Stem Cell. Oct, 2010 | Pubmed ID: 20887950 Expression from a Betageo Gene Trap in the Slain1 Gene Locus is Predominantly Associated with the Developing Nervous System The International Journal of Developmental Biology. 2010 | Pubmed ID: 20563991 Differentiating Embryonic Stem Cells Pass Through 'temporal Windows' That Mark Responsiveness to Exogenous and Paracrine Mesendoderm Inducing Signals PloS One. 2010 | Pubmed ID: 20502661 Fourier Transform Infrared Microspectroscopy Identifies Early Lineage Commitment in Differentiating Human Embryonic Stem Cells Stem Cell Research. Mar, 2010 | Pubmed ID: 20060373 Generation of Human Embryonic Stem Cell Reporter Knock-in Lines by Homologous Recombination Current Protocols in Stem Cell Biology. Nov, 2009 | Pubmed ID: 19885825 Reshaping Pluripotent Stem Cells Nature Biotechnology. Sep, 2009 | Pubmed ID: 19741639 ErythRED, a HESC Line Enabling Identification of Erythroid Cells Nature Methods. Sep, 2009 | Pubmed ID: 19701193 Pancreatic Differentiation from Pluripotent Stem Cells: Tweaking the System Cell Research. Apr, 2009 | Pubmed ID: 19339975 Enforced Expression of Mixl1 During Mouse ES Cell Differentiation Suppresses Hematopoietic Mesoderm and Promotes Endoderm Formation Stem Cells (Dayton, Ohio). Feb, 2009 | Pubmed ID: 19038793 A Protocol for Removal of Antibiotic Resistance Cassettes from Human Embryonic Stem Cells Genetically Modified by Homologous Recombination or Transgenesis Nature Protocols. 2008 | Pubmed ID: 18802436 Directed Differentiation of Human Embryonic Stem Cells As Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay Current Protocols in Stem Cell Biology. Jan, 2008 | Pubmed ID: 18770631 Expansion of Human Embryonic Stem Cells in Vitro Current Protocols in Stem Cell Biology. May, 2008 | Pubmed ID: 18770627 A Protocol Describing the Use of a Recombinant Protein-based, Animal Product-free Medium (APEL) for Human Embryonic Stem Cell Differentiation As Spin Embryoid Bodies Nature Protocols. 2008 | Pubmed ID: 18451785 Building Better Beta Cells Cell Stem Cell. Apr, 2008 | Pubmed ID: 18397747 Targeting a GFP Reporter Gene to the MIXL1 Locus of Human Embryonic Stem Cells Identifies Human Primitive Streak-like Cells and Enables Isolation of Primitive Hematopoietic Precursors Blood. Feb, 2008 | Pubmed ID: 18032708 Endocrine Cells Develop Within Pancreatic Bud-like Structures Derived from Mouse ES Cells Differentiated in Response to BMP4 and Retinoic Acid Stem Cell Research. Oct, 2007 | Pubmed ID: 19383384 Inaugural Issue of Stem Cell Research Stem Cell Research. Oct, 2007 | Pubmed ID: 19383380 Pancreas Differentiation of Mouse ES Cells Current Protocols in Stem Cell Biology. Aug, 2007 | Pubmed ID: 18785172 Differentiation of Human Embryonic Stem Cells in Serum-free Medium Reveals Distinct Roles for Bone Morphogenetic Protein 4, Vascular Endothelial Growth Factor, Stem Cell Factor, and Fibroblast Growth Factor 2 in Hematopoiesis Stem Cells (Dayton, Ohio). Sep, 2007 | Pubmed ID: 17556598 A Method for Genetic Modification of Human Embryonic Stem Cells Using Electroporation Nature Protocols. 2007 | Pubmed ID: 17446878 A Mouse Carrying the Green Fluorescent Protein Gene Targeted to the Pdx1 Locus Facilitates the Study of Pancreas Development and Function Genesis (New York, N.Y. : 2000). Jun, 2006 | Pubmed ID: 16794995 Transcriptional Profiling of Mouse and Human ES Cells Identifies SLAIN1, a Novel Stem Cell Gene Developmental Biology. May, 2006 | Pubmed ID: 16546155 Mixl1 and Oct4 Proteins Are Transiently Co-expressed in Differentiating Mouse and Human Embryonic Stem Cells Stem Cells and Development. Dec, 2005 | Pubmed ID: 16433620 Forced Aggregation of Defined Numbers of Human Embryonic Stem Cells into Embryoid Bodies Fosters Robust, Reproducible Hematopoietic Differentiation Blood. Sep, 2005 | Pubmed ID: 15914555 The HESC Line Envy Expresses High Levels of GFP in All Differentiated Progeny Nature Methods. Apr, 2005 | Pubmed ID: 15782217 SCL/Tal-1 is Essential for Hematopoietic Commitment of the Hemangioblast but Not for Its Development Blood. May, 2005 | Pubmed ID: 15677567 The Primitive Streak Gene Mixl1 is Required for Efficient Haematopoiesis and BMP4-induced Ventral Mesoderm Patterning in Differentiating ES Cells Development (Cambridge, England). Mar, 2005 | Pubmed ID: 15673572 Retinoic Acid Induces Pdx1-positive Endoderm in Differentiating Mouse Embryonic Stem Cells Diabetes. Feb, 2005 | Pubmed ID: 15585742 The Critical Regulator of Embryonic Hematopoiesis, SCL, is Vital in the Adult for Megakaryopoiesis, Erythropoiesis, and Lineage Choice in CFU-S12 Proceedings of the National Academy of Sciences of the United States of America. Feb, 2003 | Pubmed ID: 12552125 Mixl1 is Required for Axial Mesendoderm Morphogenesis and Patterning in the Murine Embryo Development (Cambridge, England). Aug, 2002 | Pubmed ID: 12117810 Characterization of Interactions Between Transcription Factors and a Regulatory Region Spanning Nt -320 to -281 of the HIV-1 LTR in T-lymphoid and Non-T-lymphoid Cells Journal of Biomedical Science. Jan-Feb, 2002 | Pubmed ID: 11810027 Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting Zerina Lokmic1,2, Elizabeth S. Ng1,3, Matthew Burton1, Edouard G. Stanley1,2,3, Anthony J. Penington1,2, Andrew G. Elefanty1,2,3 1Murdoch Childrens Research Institute, The Royal Childrens Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton JoVE 52691 Medicina
Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting Zerina Lokmic1,2, Elizabeth S. Ng1,3, Matthew Burton1, Edouard G. Stanley1,2,3, Anthony J. Penington1,2, Andrew G. Elefanty1,2,3 1Murdoch Childrens Research Institute, The Royal Childrens Hospital, 2Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, 3Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton JoVE 52691 Medicina