Jan Huisken Multiscale Biology Georg-August-University Göttingen Biography Publications Institution JoVE Articles Jan Huisken Jan Huisken is a Alexander-von-Humboldt Professor at the Georg-August University in Göttingen. Jan studied physics in Göttingen and Heidelberg and has a background in three-dimensional fluorescence microscopy, optical manipulation and trapping, developmental biology and zebrafish development. He received his PhD from the EMBL Heidelberg, where he pioneered multidimensional light sheet microscopy (also Selective Plane Illumination Microscopy, SPIM) in the labs of Ernst Stelzer and Joachim Wittbrodt. For one of the first applications of light sheet microscopy, Huisken moved to the lab of Didier Stainier at the University of California San Francisco as a cross-disciplinary HFSP postdoctoral fellow in 2005 to study cardiovascular morphogenesis and function in zebrafish. From 2010 until 2016 Huisken was an independent group leader at the Max Planck Institute for Molecular Cell Biology and Genetics in Dresden, Germany. From 2016 until 2021 he was a principal investigator and director of Medical Engineering at the Morgridge Institute for Research and Professor at the University of Wisconsin Madison. Huisken is now best known for his interdisciplinary work at the interface of gentle high-resolution microscopy and quantitative developmental biology. His lab covers all aspects of modern in vivo imaging from sample preparation to image analysis. Recently he has started an initiative to democratize the access to advanced microscopy with a modular and portable microscope platform called Flamingo. For his contributions to modern optical microscopy Huisken was awarded the Royal Microscopy Society Medal for Light Microscopy in 2017, the Lennart Nilsson Award in 2020, and the Humboldt-Professorship in 2021. Publications Tutorial: Practical Considerations for Tissue Clearing and Imaging Nature Protocols. 06, 2021 | Pubmed ID: 34021294 Image Quality Guided Smart Rotation Improves Coverage in Microscopy Nature Communications. 01, 2020 | Pubmed ID: 31919345 Multi-scale Imaging and Analysis Identify Pan-embryo Cell Dynamics of Germlayer Formation in Zebrafish Nature Communications. 12, 2019 | Pubmed ID: 31848345 Putting Advanced Microscopy in the Hands of Biologists Nature Methods. 11, 2019 | Pubmed ID: 31619775 Multi-sample SPIM Image Acquisition, Processing and Analysis of Vascular Growth in Zebrafish Development (Cambridge, England). 03, 2019 | Pubmed ID: 30824551 Dynamic and Non-contact 3D Sample Rotation for Microscopy Nature Communications. 11, 2018 | Pubmed ID: 30487638 Adaptable, Illumination Patterning Light Sheet Microscopy Scientific Reports. 06, 2018 | Pubmed ID: 29941918 Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos Anjalie Schlaeppi1, Alyssa Graves1, Michael Weber1, Jan Huisken1 1Morgridge Institute for Research, Madison, WI JoVE 62741 Biologia Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish Michael Weber1, Michaela Mickoleit1, Jan Huisken1 1Huisken Lab, Max Planck Institute of Molecular Cell Biology and Genetics JoVE 51119 Biologia
Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos Anjalie Schlaeppi1, Alyssa Graves1, Michael Weber1, Jan Huisken1 1Morgridge Institute for Research, Madison, WI JoVE 62741 Biologia
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish Michael Weber1, Michaela Mickoleit1, Jan Huisken1 1Huisken Lab, Max Planck Institute of Molecular Cell Biology and Genetics JoVE 51119 Biologia