Johanna F Dekkers Research Prinsess Maxima Centre for pediatric oncology Biography Publications Institution JoVE Articles Johanna F DekkersPostdoc Florijn Dekkers works with the human organoid or ‘mini-organ’ technology to faithfully recreate healthy organs or tumors in a dish. She uses these cultures to study the effectiveness of drug treatment in a patient-specific manner. It facilitates the development of therapy that is tailored to the individual. This is of great importance for children with cancer, because they can suffer from serious treatment side effects which often establish later in life.She also combines organoids with advanced fluorescent microscopy. These methods include large-scale single cell-resolution 3D imaging on fixed tissue and 3D live-cell imaging in vitro and in vivo. She develops innovative approaches to visualize cancer biology in a unique way to thereby better understand the progression of cancer and the mechanism-of-action of anticancer therapy, such as immunotherapy with engineered T cells. She currently mainly applies her studies on breast cancer, but will translate her methods to study pediatric cancer. Publications Forskolin-induced Swelling of Intestinal Organoids Correlates with Disease Severity in Adults with Cystic Fibrosis and Homozygous F508del Mutations Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society. Nov, 2019 | Pubmed ID: 31735562 High-resolution 3D Imaging of Fixed and Cleared Organoids Nature Protocols. 06, 2019 | Pubmed ID: 31053799 Long-term Expanding Human Airway Organoids for Disease Modeling The EMBO Journal. Feb, 2019 | Pubmed ID: 30643021 Bozulmamış Organoidlerin Tek Hücreli Çözünürlükte Üç Boyutlu Görüntülemesi Ravian L. van Ineveld*1,2,3, Hendrikus C.R. Ariese*1,2,3, Ellen J. Wehrens1,2,3, Johanna F. Dekkers*1,2,3,4, Anne C. Rios*1,2,3 1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht JoVE 60709 Biologia Forskolin kaynaklı bağırsak Organoids şişme: Bir Sylvia F. Boj1, Annelotte M. Vonk2, Marvin Statia1, Jinyi Su1, Johanna F. Dekkers3, Robert R. G. Vries1, Jeffrey M. Beekman2, Hans Clevers1,4 1Foundation Hubrecht Organoid Technology, 2Department of Pediatric Pulmonology, Regenerative Medicine Centre Utrecht, Wilhelmina Children's Hospital, University Medical Centre Utrecht, 3Department of Stem Cells and Cancer, Walter and Eliza Hall Institute of Medical Research, 4Hubrecht Institute for Developmental Biology and Stem Cell Research, University Medical Centre Utrecht Medicina
Bozulmamış Organoidlerin Tek Hücreli Çözünürlükte Üç Boyutlu Görüntülemesi Ravian L. van Ineveld*1,2,3, Hendrikus C.R. Ariese*1,2,3, Ellen J. Wehrens1,2,3, Johanna F. Dekkers*1,2,3,4, Anne C. Rios*1,2,3 1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht JoVE 60709 Biologia
Forskolin kaynaklı bağırsak Organoids şişme: Bir Sylvia F. Boj1, Annelotte M. Vonk2, Marvin Statia1, Jinyi Su1, Johanna F. Dekkers3, Robert R. G. Vries1, Jeffrey M. Beekman2, Hans Clevers1,4 1Foundation Hubrecht Organoid Technology, 2Department of Pediatric Pulmonology, Regenerative Medicine Centre Utrecht, Wilhelmina Children's Hospital, University Medical Centre Utrecht, 3Department of Stem Cells and Cancer, Walter and Eliza Hall Institute of Medical Research, 4Hubrecht Institute for Developmental Biology and Stem Cell Research, University Medical Centre Utrecht Medicina