Léon Reubsaet Department of Pharmacy University of Oslo Biography Publications Institution JoVE Articles Léon Reubsaet has not added a biography. If you are Léon Reubsaet and would like to personalize this page please email our Author Liaison for assistance. Publications The Utility of Molecularly Imprinted Polymers for Mass Spectrometric Protein and Proteomics Analysis Proteomics. Dec, 2022 | Pubmed ID: 36217925 On the Spot Immunocapture in Targeted Biomarker Analysis Using Paper-bound Streptavidin As Anchor for Biotinylated Antibodies Analytical and Bioanalytical Chemistry. Aug, 2022 | Pubmed ID: 35687151 A Novel Porcine Model of Ischemia-Reperfusion Injury After Cross-Clamping the Thoracic Aorta Revealed Substantial Cardiopulmonary, Thromboinflammatory and Biochemical Changes Without Effect of C1-Inhibitor Treatment Frontiers in Immunology. 2022 | Pubmed ID: 35432333 Affinity Capture in Bottom-up Protein Analysis - Overview of Current Status of Proteolytic Peptide Capture Using Antibodies and Molecularly Imprinted Polymers Analytica Chimica Acta. Oct, 2021 | Pubmed ID: 34602193 On-line Duplex Molecularly Imprinted Solid-phase Extraction for Analysis of Low-abundant Biomarkers in Human Serum by Liquid Chromatography-tandem Mass Spectrometry Journal of Chromatography. A. Oct, 2021 | Pubmed ID: 34479097 Facilitating Serum Determination of Neuron Specific Enolase at Clinically Relevant Levels by Coupling On-line Molecularly Imprinted Solid-phase Extraction to LC-MS/MS Analytica Chimica Acta. Dec, 2020 | Pubmed ID: 33218483 Liquid Chromatography Mass Spectrometry Based Characterization of Epitope Configurations Analytical Methods : Advancing Methods and Applications. Dec, 2020 | Pubmed ID: 33141131 Magnetic Synthetic Receptors for Selective Clean-Up in Protein Biomarker Quantification Journal of Proteome Research. Aug, 2020 | Pubmed ID: 32614597 Nano Volume Fractionation Strategy for Dilute-and-shoot Injections in Off-line Loss-less Proteomic Workflows for Extensive Protein Identifications of Ultra-low Sample Amounts Journal of Chromatography. A. Jan, 2020 | Pubmed ID: 31522804 Immunocapture Sample Clean-up in Determination of Low Abundant Protein Biomarkers - a Feasibility Study of Peptide Capture by Anti-protein Antibodies RSC Advances. Oct, 2019 | Pubmed ID: 35702551 All-in-one Paper-based Sampling Chip for Targeted Protein Analysis Analytica Chimica Acta. Dec, 2019 | Pubmed ID: 31627819 Paper-based Immunocapture for Targeted Protein Analysis Talanta. Apr, 2019 | Pubmed ID: 30625614 Data-Independent Acquisition for the Orbitrap Q Exactive HF: A Tutorial Journal of Proteome Research. Mar, 2019 | Pubmed ID: 30557026 Selective Fishing for Peptides with Antibody-Immobilized Acrylate Monoliths, Coupled Online with NanoLC-MS Analytical Chemistry. Dec, 2018 | Pubmed ID: 30384595 Smart Blood Spots for Whole Blood Protein Analysis The Analyst. Jun, 2018 | Pubmed ID: 29892755 Correction To: Exploring the Peptide Retention Mechanism in Molecularly Imprinted Polymers Analytical and Bioanalytical Chemistry. Feb, 2018 | Pubmed ID: 29285643 Instant On-paper Protein Digestion During Blood Spot Sampling The Analyst. Oct, 2017 | Pubmed ID: 28895589 Exploring the Peptide Retention Mechanism in Molecularly Imprinted Polymers Analytical and Bioanalytical Chemistry. Sep, 2017 | Pubmed ID: 28752338 To Elute or Not to Elute in Immunocapture Bottom-up LC-MS Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Jun, 2017 | Pubmed ID: 28445847 Filter Plate-Based Screening of MIP SPE Materials for Capture of the Biomarker Pro-Gastrin-Releasing Peptide SLAS Discovery : Advancing Life Sciences R & D. Dec, 2017 | Pubmed ID: 28346098 Automated Protein Biomarker Analysis: On-line Extraction of Clinical Samples by Molecularly Imprinted Polymers Scientific Reports. Mar, 2017 | Pubmed ID: 28303910 Isolation and Mass Spectrometry Analysis of Urinary Extraexosomal Proteins Scientific Reports. Nov, 2016 | Pubmed ID: 27805059 Evaluation of Affinity-based Serum Clean-up in Mass Spectrometric Analysis: Plastic Vs Monoclonal Antibodies Journal of Chromatography. A. Nov, 2016 | Pubmed ID: 27743596 Dual-immuno-MS Technique for Improved Differentiation Power in Heterodimeric Protein Biomarker Analysis: Determination and Differentiation of Human Chorionic Gonadotropin Variants in Serum Analytical and Bioanalytical Chemistry. Oct, 2016 | Pubmed ID: 27485622 Cyclic Diguanylate Regulation of Bacillus Cereus Group Biofilm Formation Molecular Microbiology. Aug, 2016 | Pubmed ID: 27116468 The Pros and Cons of Increased Trypsin-to-protein Ratio in Targeted Protein Analysis Journal of Pharmaceutical and Biomedical Analysis. May, 2016 | Pubmed ID: 26907699 The Impact of High-dose Acetylcholine on Bovine Corneal Epithelium Acta Ophthalmologica. Mar, 2016 | Pubmed ID: 26448582 Vaccination with Outer Membrane Vesicles from Francisella Noatunensis Reduces Development of Francisellosis in a Zebrafish Model Fish & Shellfish Immunology. Jan, 2015 | Pubmed ID: 25449706 Peptide Imprinted Receptors for the Determination of the Small Cell Lung Cancer Associated Biomarker Progastrin Releasing Peptide Journal of Chromatography. A. Nov, 2014 | Pubmed ID: 25454129 A Pilot Study Showing Differences in Glycosylation Patterns of IgG Subclasses Induced by Pneumococcal, Meningococcal, and Two Types of Influenza Vaccines Immunity, Inflammation and Disease. Aug, 2014 | Pubmed ID: 25400928 Antibody-free Biomarker Determination: Exploring Molecularly Imprinted Polymers for Pro-gastrin Releasing Peptide Analytical Chemistry. Dec, 2014 | Pubmed ID: 25397409 Why Less is More when Generating Tryptic Peptides in Bottom-up Proteomics Proteomics. Sep, 2014 | Pubmed ID: 25044798 Multiplexing Determination of Small Cell Lung Cancer Biomarkers and Their Isovariants in Serum by Immunocapture LC-MS/MS Analytical Chemistry. Jul, 2014 | Pubmed ID: 24945626 Determining ProGRP and Isoforms in Lung and Thyroid Cancer Patient Samples: Comparing an MS Method with a Routine Clinical Immunoassay Analytical and Bioanalytical Chemistry. Apr, 2014 | Pubmed ID: 24518900 Bradykinin Analysis Revived--a Validated Method for Determination of Its Stable Metabolite in Whole Blood by LC-MS/MS Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Feb, 2014 | Pubmed ID: 24424302 Epitope Analysis and Detection of Human Chorionic Gonadotropin (hCG) Variants by Monoclonal Antibodies and Mass Spectrometry Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine. Feb, 2014 | Pubmed ID: 24014048 Integrated Enzyme Reactor and High Resolving Chromatography in "sub-chip" Dimensions for Sensitive Protein Mass Spectrometry Scientific Reports. Dec, 2013 | Pubmed ID: 24336509 Immunocapture and LC-MS/MS for Selective Quantification and Differentiation of the Isozymes of the Biomarker Neuron-specific Enolase in Serum Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Jun, 2013 | Pubmed ID: 23669612 Comparison of Newly Developed Immuno-MS Method with Existing DELFIA(®) Immunoassay for Human Chorionic Gonadotropin Determination in Doping Analysis Bioanalysis. Mar, 2013 | Pubmed ID: 23425277 Sports Drug Testing Using Immuno-MS: Clinical Study Comprising Administration of Human Chorionic Gonadotropin to Males Analytical and Bioanalytical Chemistry. Feb, 2013 | Pubmed ID: 23203507 Digging Deeper into the Field of the Small Cell Lung Cancer Tumor Marker ProGRP: a Method for Differentiation of Its Isoforms Journal of Proteome Research. Jan, 2013 | Pubmed ID: 23190087 Urinary Proteomic Shotgun Approach for Identification of Potential Acute Rejection Biomarkers in Renal Transplant Recipients Transplantation Research. Aug, 2012 | Pubmed ID: 23369437 Immuno-MS Based Targeted Proteomics: Highly Specific, Sensitive, and Reproducible Human Chorionic Gonadotropin Determination for Clinical Diagnostics and Doping Analysis Analytical Chemistry. Sep, 2012 | Pubmed ID: 22894612 Fast, Selective, and Sensitive Analysis of Low-abundance Peptides in Human Plasma by Electromembrane Extraction Analytica Chimica Acta. Feb, 2012 | Pubmed ID: 22284872 Critical Assessment of Accelerating Trypsination Methods Journal of Pharmaceutical and Biomedical Analysis. Dec, 2011 | Pubmed ID: 21873015 Fundamental Studies on the Electrokinetic Transfer of Net Cationic Peptides Across Supported Liquid Membranes Journal of Separation Science. Jan, 2011 | Pubmed ID: 21246724 Elucidation of Phosphatidylcholine Composition in Krill Oil Extracted from Euphausia Superba Lipids. Jan, 2011 | Pubmed ID: 20848234 Accelerated 18O-labeling in Urinary Proteomics Journal of Chromatography. A. Dec, 2010 | Pubmed ID: 21094492 Potential-driven Peptide Extractions Across Supported Liquid Membranes: Investigation of Principal Operational Parameters Journal of Separation Science. Jun, 2010 | Pubmed ID: 20437412 2-D Hydrophilic Interaction Liquid Chromatography-RP Separation in Urinary Proteomics--minimizing Variability Through Improved Downstream Workflow Compatibility Journal of Separation Science. Mar, 2010 | Pubmed ID: 20024930 Rapid Isolation of Angiotensin Peptides from Plasma by Electromembrane Extraction Journal of Chromatography. A. Oct, 2009 | Pubmed ID: 19720379 Exploring the Complementary Selectivity of Immunocapture and MS Detection for the Differentiation Between HCG Isoforms in Clinically Relevant Samples Journal of Proteome Research. Nov, 2009 | Pubmed ID: 19719329 Immuno-capture As Ultimate Sample Cleanup in LC-MS/MS Determination of the Early Stage Biomarker ProGRP Journal of Separation Science. Sep, 2009 | Pubmed ID: 19642102 Absolute ProGRP Quantification in a Clinical Relevant Concentration Range Using LC-MS/MS and a Comprehensive Internal Standard Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. May, 2009 | Pubmed ID: 19124278 On-Line Multitasking Analytical Proteomics: How to Separate, Reduce, Alkylate and Digest Whole Proteins in an On-Line Multidimensional Chromatography System Coupled to MS Journal of Separation Science. Sep, 2008 | Pubmed ID: 18704998 Improving Off-line Accelerated Tryptic Digestion. Towards Fast-lane Proteolysis of Complex Biological Samples Journal of Chromatography. A. Jun, 2008 | Pubmed ID: 18502436 Electromembrane Extraction of Peptides Journal of Chromatography. A. Jun, 2008 | Pubmed ID: 18479691 Targeted Determination of the Early Stage SCLC Specific Biomarker Pro-gastrin-releasing Peptide (ProGRP) at Clinical Concentration Levels in Human Serum Using LC-MS Journal of Separation Science. Nov, 2007 | Pubmed ID: 17874417 Determination of Ganciclovir in Different Matrices from Solid Organ Transplanted Patients Treated with a Wide Range of Concomitant Drugs Journal of Pharmaceutical and Biomedical Analysis. Feb, 2007 | Pubmed ID: 17034976 Acetylcholine in the Corneal Epithelium of Diurnal and Nocturnal Mammals Cornea. Nov, 2005 | Pubmed ID: 16227851 Ion-pair Mediated Transport of Angiotensin, Neurotensin, and Their Metabolites in Liquid Phase Microextraction Under Acidic Conditions Journal of Separation Science. Jul, 2005 | Pubmed ID: 16116998 Application of Supplementary Flow in Comprehensive 2D Liquid Chromatography Combining SEC and RPC Journal of Separation Science. Mar, 2005 | Pubmed ID: 15835736 Ion-pair Mediated Transport of Small Model Peptides in Liquid Phase Micro Extraction Under Acidic Conditions Journal of Separation Science. Feb, 2005 | Pubmed ID: 15776934 Screening for Central Nervous System-stimulating Drugs in Human Plasma by Liquid Chromatography with Mass Spectrometric Detection Journal of Chromatography. A. Mar, 2004 | Pubmed ID: 15058584 Three-phase Liquid-phase Microextraction of Weakly Basic Drugs from Whole Blood Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences. Dec, 2003 | Pubmed ID: 14630367 High-performance Liquid Chromatography-mass Spectrometry Analysis of Diltiazem and 11 of Its Phase I Metabolites in Human Plasma Journal of Pharmaceutical and Biomedical Analysis. Sep, 2003 | Pubmed ID: 12972092 Sample Preparation and Determination of Acetylcholine in Corneal Epithelium Cells Using Liquid Chromatography-tandem Mass Spectrometry Journal of Chromatographic Science. Mar, 2003 | Pubmed ID: 12725699 Pharmacokinetics of Diltiazem and Its Metabolites in Relation to CYP2D6 Genotype Clinical Pharmacology and Therapeutics. Sep, 2002 | Pubmed ID: 12235455 Micromuestreo en análisis de proteínas basado en espectrometría de masas dirigida de proteínas de baja abundancia Huan Bao Ngo1, Inger Oulie1, Léon Reubsaet1, Trine Grønhaug Halvorsen1 1Department of Pharmacy, University of Oslo JoVE 64473 Chimica
Micromuestreo en análisis de proteínas basado en espectrometría de masas dirigida de proteínas de baja abundancia Huan Bao Ngo1, Inger Oulie1, Léon Reubsaet1, Trine Grønhaug Halvorsen1 1Department of Pharmacy, University of Oslo JoVE 64473 Chimica