Nicola A. Burgess-Brown Centre for Medicines Discovery, Nuffield Department of Medicine University of Oxford Biography Publications Institution JoVE Articles Nicola A. Burgess-Brown has not added a biography. If you are Nicola A. Burgess-Brown and would like to personalize this page please email our Author Liaison for assistance. Publications Cryo-EM Structure of the Human Kv3.1 Channel Reveals Gating Control by the Cytoplasmic T1 Domain Nature Communications. Jul, 2022 | Pubmed ID: 35840580 FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult Recombinant Proteins in S2 Cells Frontiers in Bioengineering and Biotechnology. 2022 | Pubmed ID: 35600892 Identification, Mapping and Relative Quantitation of SARS-CoV-2 Spike Glycopeptides by Mass-Retention Time Fingerprinting Communications Biology. Aug, 2021 | Pubmed ID: 34345007 Beyond Just Peptide Antigens: The Complex World of Peptide-Based Cancer Vaccines Frontiers in Immunology. 2021 | Pubmed ID: 34276688 Selection and Structural Characterization of Anti-TREM2 ScFvs That Reduce Levels of Shed Ectodomain Structure (London, England : 1993). Nov, 2021 | Pubmed ID: 34233201 The Structural Basis of Fatty Acid Elongation by the ELOVL Elongases Nature Structural & Molecular Biology. Jun, 2021 | Pubmed ID: 34117479 Phospho-regulation, Nucleotide Binding and Ion Access Control in Potassium-chloride Cotransporters The EMBO Journal. Jul, 2021 | Pubmed ID: 34031912 Structure and Activation Mechanism of the Human Liver-type Glutaminase GLS2 Biochimie. Jun, 2021 | Pubmed ID: 33746066 Expression Screening of Human Integral Membrane Proteins Using BacMam Methods in Molecular Biology (Clifton, N.J.). 2021 | Pubmed ID: 33125646 Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells Methods in Molecular Biology (Clifton, N.J.). 2021 | Pubmed ID: 33125645 Screening and Production of Recombinant Human Proteins: Protein Production in E. Coli Methods in Molecular Biology (Clifton, N.J.). 2021 | Pubmed ID: 33125644 Screening and Production of Recombinant Human Proteins: Ligation-Independent Cloning Methods in Molecular Biology (Clifton, N.J.). 2021 | Pubmed ID: 33125643 A Lower X-gate in TASK Channels Traps Inhibitors Within the Vestibule Nature. Jun, 2020 | Pubmed ID: 32499642 The Structural Basis of Lipid Scrambling and Inactivation in the Endoplasmic Reticulum Scramblase TMEM16K Nature Communications. Sep, 2019 | Pubmed ID: 31477691 High-Throughput Site-Directed Mutagenesis Methods in Molecular Biology (Clifton, N.J.). 2019 | Pubmed ID: 31267458 Structures of DPAGT1 Explain Glycosylation Disease Mechanisms and Advance TB Antibiotic Design Cell. Nov, 2018 | Pubmed ID: 30388443 The SGC Beyond Structural Genomics: Redefining the Role of 3D Structures by Coupling Genomic Stratification with Fragment-based Discovery Essays in Biochemistry. Nov, 2017 | Pubmed ID: 29118096 Potent and Selective KDM5 Inhibitor Stops Cellular Demethylation of H3K4me3 at Transcription Start Sites and Proliferation of MM1S Myeloma Cells Cell Chemical Biology. Mar, 2017 | Pubmed ID: 28262558 Structure of the Polycystic Kidney Disease TRP Channel Polycystin-2 (PC2) Nature Structural & Molecular Biology. Feb, 2017 | Pubmed ID: 27991905 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors Journal of Medicinal Chemistry. Feb, 2016 | Pubmed ID: 26741168 Crystal Structure of Porphyromonas Gingivalis Peptidylarginine Deiminase: Implications for Autoimmunity in Rheumatoid Arthritis Annals of the Rheumatic Diseases. Jun, 2016 | Pubmed ID: 26209657 K2P Channel Gating Mechanisms Revealed by Structures of TREK-2 and a Complex with Prozac Science (New York, N.Y.). Mar, 2015 | Pubmed ID: 25766236 Optimisation of a Triazolopyridine Based Histone Demethylase Inhibitor Yields a Potent and Selective KDM2A (FBXL11) Inhibitor MedChemComm. Dec, 2014 | Pubmed ID: 26682034 Assessing Cellular Efficacy of Bromodomain Inhibitors Using Fluorescence Recovery After Photobleaching Epigenetics & Chromatin. 2014 | Pubmed ID: 25097667 Medium-throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 24203326 Medium-throughput Production of Recombinant Human Proteins: Protein Production in E. Coli Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 24203325 Medium-throughput Production of Recombinant Human Proteins: Ligation-independent Cloning Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 24203324 Structural Insights into Functional Overlapping and Differentiation Among Myosin V Motors The Journal of Biological Chemistry. Nov, 2013 | Pubmed ID: 24097982 Structures of ABCB10, a Human ATP-binding Cassette Transporter in Apo- and Nucleotide-bound States Proceedings of the National Academy of Sciences of the United States of America. Jun, 2013 | Pubmed ID: 23716676 The Structural Basis of ZMPSTE24-dependent Laminopathies Science (New York, N.Y.). Mar, 2013 | Pubmed ID: 23539603 The PHD and Chromo Domains Regulate the ATPase Activity of the Human Chromatin Remodeler CHD4 Journal of Molecular Biology. Sep, 2012 | Pubmed ID: 22575888 Expressing the Human Proteome for Affinity Proteomics: Optimising Expression of Soluble Protein Domains and in Vivo Biotinylation New Biotechnology. Jun, 2012 | Pubmed ID: 22027370 Human SNM1A and XPF-ERCC1 Collaborate to Initiate DNA Interstrand Cross-link Repair Genes & Development. Sep, 2011 | Pubmed ID: 21896658 A Prominent β-hairpin Structure in the Winged-helix Domain of RECQ1 is Required for DNA Unwinding and Oligomer Formation Nucleic Acids Research. Mar, 2011 | Pubmed ID: 21059676 High-throughput Production of Human Proteins for Crystallization: the SGC Experience Journal of Structural Biology. Oct, 2010 | Pubmed ID: 20541610 Large-scale Structural Analysis of the Classical Human Protein Tyrosine Phosphatome Cell. Jan, 2009 | Pubmed ID: 19167335 High Throughput Production of Recombinant Human Proteins for Crystallography Methods in Molecular Biology (Clifton, N.J.). 2008 | Pubmed ID: 18542867 Codon Optimization Can Improve Expression of Human Genes in Escherichia Coli: A Multi-gene Study Protein Expression and Purification. May, 2008 | Pubmed ID: 18289875 Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes (Video) | JoVE Sagar Raturi1, Huanyu Li1, Yung-Ning Chang2, Andreea Scacioc1, Tina Bohstedt1, Alejandra Fernandez-Cid1, Adam Evans1, Patrizia Abrusci1, Abilasha Balakrishnan1, Tomas C. Pascoa1, Didi He1, Gamma Chi1, Nanki Kaur Singh1, Mingda Ye1, Anna Li1, Leela Shrestha1, Dong Wang1, Eleanor P. Williams1, Nicola A. Burgess-Brown1, Katharina L. Dürr1, Vera Puetter2, Alvaro Ingles-Prieto3, David B. Sauer1 1Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, 2Nuvisan ICB GmbH, 3CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences JoVE 65878 Biochimica
Expression and Purification of Human Solute Carrier Transporters Using Codon-Optimized Genes (Video) | JoVE Sagar Raturi1, Huanyu Li1, Yung-Ning Chang2, Andreea Scacioc1, Tina Bohstedt1, Alejandra Fernandez-Cid1, Adam Evans1, Patrizia Abrusci1, Abilasha Balakrishnan1, Tomas C. Pascoa1, Didi He1, Gamma Chi1, Nanki Kaur Singh1, Mingda Ye1, Anna Li1, Leela Shrestha1, Dong Wang1, Eleanor P. Williams1, Nicola A. Burgess-Brown1, Katharina L. Dürr1, Vera Puetter2, Alvaro Ingles-Prieto3, David B. Sauer1 1Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, 2Nuvisan ICB GmbH, 3CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences JoVE 65878 Biochimica