Ronald J Patterson Department of Microbiology and Molecular Genetics Michigan State University Biography Publications Institution JoVE Articles Ronald J Patterson Ronald Patterson is an emeritus professor of Microbiology and Molecular Genetics at Michigan State University. He received his PhD from Northwestern University Medical School where he studied intracellular growth of Mycobacterium tuberculosis under the tutelage of Guy Youmans. He discovered lymphocytes from immune mice secreted a substance that when added to macrophages from unimmunized mice resulted in inhibition of intracellular mycobacterial growth.His postdoctoral training under Brian McCarthy at the University of Washington turned to the molecular aspects of determining the number of antibody genes via mRNA hybridization techniques when this was a major question in molecular genetics.After this entry into mRNA biology, Dr Patterson took a position at Michigan State in the Department of Microbiology and studied the regulation of mRNA transport from nuclei using an in vitro isolated nuclei system. His group validated this model showing that introns of immunoglobulin genes are retained in nuclei while exons are transported.Following a sabbatical with Thoru Pederson investigating mRNA splicing, he returned to Michigan State and initiated a long term collaboration with John Wang establishing the essential role of galectin-1 and -3 in pre-mRNA splicing. As this paper demonstrates, gal-3 is recruited to the assembling spliceosome through its association with U1 snRNP. Publications A 10S Galectin-3-U1 SnRNP Complex Assembles into Active Spliceosomes Nucleic Acids Research. 07, 2016 | Pubmed ID: 27105840 Examination of the Role of Galectins in Pre-mRNA Splicing Methods in Molecular Biology (Clifton, N.J.). Month, 2015 | Pubmed ID: 25253157 SR Proteins and Galectins: What's in a Name? Glycobiology. Oct, 2010 | Pubmed ID: 20574110 Dynamics of Galectin-3 in the Nucleus and Cytoplasm Biochimica Et Biophysica Acta. Feb, 2010 | Pubmed ID: 19616076 A Mechanism for Incorporation of Galectin-3 into the Spliceosome Through Its Association with U1 SnRNP Biochemistry. Aug, 2009 | Pubmed ID: 19603745 Dissociation of the Carbohydrate-binding and Splicing Activities of Galectin-1 Archives of Biochemistry and Biophysics. Oct, 2008 | Pubmed ID: 18662664 Distinct Effects on Splicing of Two Monoclonal Antibodies Directed Against the Amino-terminal Domain of Galectin-3 Archives of Biochemistry and Biophysics. Jul, 2008 | Pubmed ID: 18455493 Immunoprecipitation of Spliceosomal RNAs by Antisera to Galectin-1 and Galectin-3 Nucleic Acids Research. Month, 2006 | Pubmed ID: 16998182 Transport of Galectin-3 Between the Nucleus and Cytoplasm. I. Conditions and Signals for Nuclear Import Glycobiology. Jul, 2006 | Pubmed ID: 16473835 Transport of Galectin-3 Between the Nucleus and Cytoplasm. II. Identification of the Signal for Nuclear Export Glycobiology. Jul, 2006 | Pubmed ID: 16473834 Nucleocytoplasmic Lectins Biochimica Et Biophysica Acta. Jul, 2004 | Pubmed ID: 15238251 Understanding the Biochemical Activities of Galectin-1 and Galectin-3 in the Nucleus Glycoconjugate Journal. Month, 2002 | Pubmed ID: 14758073 Shuttling of Galectin-3 Between the Nucleus and Cytoplasm Glycobiology. May, 2002 | Pubmed ID: 12070075 Association of Galectin-1 and Galectin-3 with Gemin4 in Complexes Containing the SMN Protein Nucleic Acids Research. Sep, 2001 | Pubmed ID: 11522829 Komplementierung der Spleißaktivität durch einen Galectin-3 - U1 snRNP Komplex auf Perlen Patricia G. Voss1, Kevin C. Haudek1, Ronald J. Patterson2, John L. Wang1 1Department of Biochemistry and Molecular Biology, Michigan State University, 2Department of Microbiology and Molecular Genetics, Michigan State University JoVE 61990 Biochimica
Komplementierung der Spleißaktivität durch einen Galectin-3 - U1 snRNP Komplex auf Perlen Patricia G. Voss1, Kevin C. Haudek1, Ronald J. Patterson2, John L. Wang1 1Department of Biochemistry and Molecular Biology, Michigan State University, 2Department of Microbiology and Molecular Genetics, Michigan State University JoVE 61990 Biochimica