Sandra Johnen Department of Ophthalmology University Hospital RWTH Aachen Biography Publications Institution JoVE Articles Sandra Johnen has not added a biography. If you are Sandra Johnen and would like to personalize this page please email our Author Liaison for assistance. Publications Establishment and Characterization of a Unilateral UV-Induced Photoreceptor Degeneration Model in the C57Bl/6J Mouse Translational Vision Science & Technology. 08, 2020 | Pubmed ID: 32879777 The Very Large Electrode Array for Retinal Stimulation (VLARS)-A Concept Study Journal of Neural Engineering. 11, 2019 | Pubmed ID: 31480027 Cryopreservation of Amniotic Membrane with and Without Glycerol Additive Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie. Jun, 2018 | Pubmed ID: 29623460 Going Non-viral: the Sleeping Beauty Transposon System Breaks on Through to the Clinical Side Critical Reviews in Biochemistry and Molecular Biology. 08, 2017 | Pubmed ID: 28402189 Engineering of PEDF-Expressing Primary Pigment Epithelial Cells by the SB Transposon System Delivered by PFAR4 Plasmids Molecular Therapy. Nucleic Acids. Mar, 2017 | Pubmed ID: 28325297 Properties of Retinal Precursor Cells Grown on Vertically Aligned Multiwalled Carbon Nanotubes Generated for the Modification of Retinal Implant-Embedded Microelectrode Arrays Journal of Ophthalmology. 2016 | Pubmed ID: 27200182 Antiangiogenic and Neurogenic Activities of Sleeping Beauty-Mediated PEDF-Transfected RPE Cells In Vitro and In Vivo BioMed Research International. 2015 | Pubmed ID: 26697494 Preclinical Evaluation of a Cell-Based Gene Therapy Using the Sleeping Beauty Transposon System in Choroidal Neovascularization Molecular Therapy. Methods & Clinical Development. | Pubmed ID: 31890733 The Antibiotic-free PFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells Molecular Therapy. Nucleic Acids. | Pubmed ID: 29858090 Long-Term PEDF Release in Rat Iris and Retinal Epithelial Cells After Sleeping Beauty Transposon-Mediated Gene Delivery Molecular Therapy. Nucleic Acids. | Pubmed ID: 29246287 Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy Thais Bascuas*1,2, Martina Kropp*1,2, Nina Harmening1,2, Brittany M. Wong1, Sandra Johnen3, Zsuzsanna Izsvák4, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Department of Ophthalmology, University Hospital RWTH Aachen, 4Max Delbrück Center for Molecular Medicine JoVE 62145 Biologia Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System Sandra Johnen1, Nina Harmening2,3, Corinne Marie4,5, Daniel Scherman4, Zsuzsanna Izsvák6, Zoltán Ivics7, Peter Walter1, Gabriele Thumann2,3 1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute JoVE 61987 Medicina
Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy Thais Bascuas*1,2, Martina Kropp*1,2, Nina Harmening1,2, Brittany M. Wong1, Sandra Johnen3, Zsuzsanna Izsvák4, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Department of Ophthalmology, University Hospital RWTH Aachen, 4Max Delbrück Center for Molecular Medicine JoVE 62145 Biologia
Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System Sandra Johnen1, Nina Harmening2,3, Corinne Marie4,5, Daniel Scherman4, Zsuzsanna Izsvák6, Zoltán Ivics7, Peter Walter1, Gabriele Thumann2,3 1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute JoVE 61987 Medicina