Silas J. Leavesley Department of Chemical and Biomolecular Engineering, College of Engineering University of South Alabama Biography Publications Institution JoVE Articles Silas J. Leavesley Dr. Silas Leavesley is a professor of Chemical and Biomolecular Engineering and a member of the Center for Lung Biology at the University of South Alabama. His research focuses on the design and implementation of hyperspectral imaging technology for preclinical and clinical diagnostics – including microscopy and endoscopy platforms. Publications Label-free Spectroscopic Tissue Characterization Using Fluorescence Excitation-scanning Spectral Imaging Journal of Biophotonics. 02, 2020 | Pubmed ID: 31566889 Applications and Assessment of an Excitation-scanning Hyperspectral Imaging System Proceedings of SPIE--the International Society for Optical Engineering. Jan-Feb, 2018 | Pubmed ID: 31762531 Identifying Molecular Contributors to Autofluorescence of Neoplastic and Normal Colon Sections Using Excitation-scanning Hyperspectral Imaging Journal of Biomedical Optics. 12, 2018 | Pubmed ID: 30592190 Spectral Imaging of FRET-based Sensors Reveals Sustained CAMP Gradients in Three Spatial Dimensions Cytometry. Part A : the Journal of the International Society for Analytical Cytology. 10, 2018 | Pubmed ID: 30176184 Feasibility of Using Thermal Response to K Band Millimeter Wave Heating to Assess Skin Blood Flow Physiological Measurement. Apr, 2018 | Pubmed ID: 29513271 Human ASIC1a Mediates Stronger Acid-induced Responses As Compared with Mouse ASIC1a FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Jul, 2018 | Pubmed ID: 29447005 A Theoretical-experimental Methodology for Assessing the Sensitivity of Biomedical Spectral Imaging Platforms, Assays, and Analysis Methods Journal of Biophotonics. Jan, 2018 | Pubmed ID: 28485093 Hyperspectral Imaging Fluorescence Excitation Scanning for Colon Cancer Detection Journal of Biomedical Optics. 10, 2016 | Pubmed ID: 27792808 Overcoming Limitations of FRET Measurements Cytometry. Part A : the Journal of the International Society for Analytical Cytology. Apr, 2016 | Pubmed ID: 27101317 Estimating the Magnitude of Near-membrane PDE4 Activity in Living Cells American Journal of Physiology. Cell Physiology. Sep, 2015 | Pubmed ID: 26201952 Channel-based Reporters for CAMP Detection Methods in Molecular Biology (Clifton, N.J.). Month, 2015 | Pubmed ID: 25783878 Automated Image Analysis of FRET Signals for Subcellular CAMP Quantification Methods in Molecular Biology (Clifton, N.J.). Month, 2015 | Pubmed ID: 25783877 FRET: Signals Hidden Within the Noise Cytometry. Part A : the Journal of the International Society for Analytical Cytology. Nov, 2014 | Pubmed ID: 25241962 A Device for Performing Automated Balloon Catheter Inflation Ischemia Studies PloS One. Month, 2014 | Pubmed ID: 24769885 Excitation-scanning Hyperspectral Imaging Microscope Journal of Biomedical Optics. Apr, 2014 | Pubmed ID: 24727909 Can We Decipher the Information Content Contained Within Cyclic Nucleotide Signals? The Journal of General Physiology. Jan, 2014 | Pubmed ID: 24378904 Thin-film Tunable Filters for Hyperspectral Fluorescence Microscopy Journal of Biomedical Optics. Jan, 2014 | Pubmed ID: 24077519 Assessing FRET Using Spectral Techniques Cytometry. Part A : the Journal of the International Society for Analytical Cytology. Oct, 2013 | Pubmed ID: 23929684 An Approach for Characterizing and Comparing Hyperspectral Microscopy Systems Sensors (Basel, Switzerland). Jul, 2013 | Pubmed ID: 23877125 Hyperspectral Imaging of FRET-based CGMP Probes Methods in Molecular Biology (Clifton, N.J.). Month, 2013 | Pubmed ID: 23709027 Assessment of Cellular Mechanisms Contributing to CAMP Compartmentalization in Pulmonary Microvascular Endothelial Cells American Journal of Physiology. Cell Physiology. Mar, 2012 | Pubmed ID: 22116306 Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis Naga S. Annamdevula1,2, Rachel Sweat3, Hayden Gunn1, John R. Griswold3, Andrea L. Britain1,2, Thomas C. Rich1,2, Silas J. Leavesley1,2,3 1Department of Pharmacology, University of South Alabama, 2Center for Lung Biology, University of South Alabama, 3Department of Chemical and Biomolecular Engineering, University of South Alabama JoVE 61720 Biologia
Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis Naga S. Annamdevula1,2, Rachel Sweat3, Hayden Gunn1, John R. Griswold3, Andrea L. Britain1,2, Thomas C. Rich1,2, Silas J. Leavesley1,2,3 1Department of Pharmacology, University of South Alabama, 2Center for Lung Biology, University of South Alabama, 3Department of Chemical and Biomolecular Engineering, University of South Alabama JoVE 61720 Biologia