Steve Oh Bioprocessing Technology Institute (BTI) Agency for Science, Technology and Research (A STAR) Biography Publications Institution JoVE Articles Steve Oh has not added a biography. If you are Steve Oh and would like to personalize this page please email our Author Liaison for assistance. Publications Selection of O-negative Induced Pluripotent Stem Cell Clones for High-density Red Blood Cell Production in a Scalable Perfusion Bioreactor System Cell Proliferation. Aug, 2022 | Pubmed ID: 35289971 Strategies to Enhance Immunomodulatory Properties and Reduce Heterogeneity in Mesenchymal Stromal Cells During Ex Vivo Expansion Cytotherapy. May, 2022 | Pubmed ID: 35227601 Integrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 34519977 Industrially Compatible Transfusable IPSC-Derived RBCs: Progress, Challenges and Prospective Solutions International Journal of Molecular Sciences. Sep, 2021 | Pubmed ID: 34575977 Multiomics Analyses of Cytokines, Genes, MiRNA, and Regulatory Networks in Human Mesenchymal Stem Cells Expanded in Stirred Microcarrier-spinner Cultures Stem Cell Research. May, 2021 | Pubmed ID: 33676128 A Scalable Suspension Platform for Generating High-Density Cultures of Universal Red Blood Cells from Human Induced Pluripotent Stem Cells Stem Cell Reports. Jan, 2021 | Pubmed ID: 33306988 Machine Learning to Predict Mesenchymal Stem Cell Efficacy for Cartilage Repair PLoS Computational Biology. Oct, 2020 | Pubmed ID: 33027251 Selection of Human Induced Pluripotent Stem Cells Lines Optimization of Cardiomyocytes Differentiation in an Integrated Suspension Microcarrier Bioreactor Stem Cell Research & Therapy. Mar, 2020 | Pubmed ID: 32183888 Human Mesenchymal Stem Cell Therapy for Cartilage Repair: Review on Isolation, Expansion, and Constructs Stem Cell Research. Apr, 2020 | Pubmed ID: 32109723 Rapid Detection of Senescent Mesenchymal Stromal Cells by a Fluorescent Probe Biotechnology Journal. Oct, 2019 | Pubmed ID: 31218816 Sub-confluent Culture of Human Mesenchymal Stromal Cells on Biodegradable Polycaprolactone Microcarriers Enhances Bone Healing of Rat Calvarial Defect Cytotherapy. Jun, 2019 | Pubmed ID: 30975604 Scaled-Up Inertial Microfluidics: Retention System for Microcarrier-Based Suspension Cultures Biotechnology Journal. May, 2019 | Pubmed ID: 30791214 Review: In Vitro Generation of Red Blood Cells for Transfusion Medicine: Progress, Prospects and Challenges Biotechnology Advances. Dec, 2018 | Pubmed ID: 30273713 Inertial-Based Filtration Method for Removal of Microcarriers from Mesenchymal Stem Cell Suspensions Scientific Reports. Aug, 2018 | Pubmed ID: 30127526 Meticulous Optimization of Cardiomyocyte Yields in a 3-stage Continuous Integrated Agitation Bioprocess Stem Cell Research. Aug, 2018 | Pubmed ID: 30098478 Unraveling the Inconsistencies of Cardiac Differentiation Efficiency Induced by the GSK3β Inhibitor CHIR99021 in Human Pluripotent Stem Cells Stem Cell Reports. Jun, 2018 | Pubmed ID: 29706502 Improved Erythroid Differentiation of Multiple Human Pluripotent Stem Cell Lines in Microcarrier Culture by Modulation of Wnt/β-Catenin Signaling Haematologica. Jul, 2018 | Pubmed ID: 29519863 Immobilization of Vitronectin-binding Heparan Sulfates Onto Surfaces to Support Human Pluripotent Stem Cells Journal of Biomedical Materials Research. Part B, Applied Biomaterials. Jul, 2018 | Pubmed ID: 28941021 Erratum: Identification of Na/K-ATPase Inhibition-independent Proarrhythmic Ionic Mechanisms of Cardiac Glycosides Scientific Reports. Sep, 2017 | Pubmed ID: 28904341 Identification of Na+/K+-ATPase Inhibition-independent Proarrhythmic Ionic Mechanisms of Cardiac Glycosides Scientific Reports. May, 2017 | Pubmed ID: 28550304 Critical Attributes of Human Early Mesenchymal Stromal Cell-laden Microcarrier Constructs for Improved Chondrogenic Differentiation Stem Cell Research & Therapy. May, 2017 | Pubmed ID: 28482913 Tunable Volumetric Density and Porous Structure of Spherical Poly-ε-caprolactone Microcarriers, As Applied in Human Mesenchymal Stem Cell Expansion Langmuir : the ACS Journal of Surfaces and Colloids. Mar, 2017 | Pubmed ID: 28221044 Biodegradable Poly-ε-caprolactone Microcarriers for Efficient Production of Human Mesenchymal Stromal Cells and Secreted Cytokines in Batch and Fed-batch Bioreactors Cytotherapy. Mar, 2017 | Pubmed ID: 28017598 Biodegradable ECM-coated PCL Microcarriers Support Scalable Human Early MSC Expansion and in Vivo Bone Formation Cytotherapy. Oct, 2016 | Pubmed ID: 27503763 Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions for Enhancing Cardiomyogenesis Efficiency of HESC Cultures Scientific Reports. Aug, 2016 | Pubmed ID: 27501774 Expansion in Microcarrier-spinner Cultures Improves the Chondrogenic Potential of Human Early Mesenchymal Stromal Cells Cytotherapy. Jun, 2016 | Pubmed ID: 27173750 International Society for Cellular Therapy Perspective on Immune Functional Assays for Mesenchymal Stromal Cells As Potency Release Criterion for Advanced Phase Clinical Trials Cytotherapy. Feb, 2016 | Pubmed ID: 26724220 Recent Advances in Serum-free Microcarrier Expansion of Mesenchymal Stromal Cells: Parameters to Be Optimized Biochemical and Biophysical Research Communications. May, 2016 | Pubmed ID: 26385177 Integrated Processes for Expansion and Differentiation of Human Pluripotent Stem Cells in Suspended Microcarriers Cultures Biochemical and Biophysical Research Communications. May, 2016 | Pubmed ID: 26385176 Enhanced in Vitro Osteogenic Differentiation of Human Fetal MSCs Attached to 3D Microcarriers Versus Harvested from 2D Monolayers BMC Biotechnology. Oct, 2015 | Pubmed ID: 26520400 Fabrication of Uniform-sized Poly-ɛ-caprolactone Microspheres and Their Applications in Human Embryonic Stem Cell Culture Biomedical Microdevices. Dec, 2015 | Pubmed ID: 26458560 Serum-free Media Formulations Are Cell Line-specific and Require Optimization for Microcarrier Culture Cytotherapy. Aug, 2015 | Pubmed ID: 26139547 Enabling Consistency in Pluripotent Stem Cell-derived Products for Research and Development and Clinical Applications Through Material Standards Stem Cells Translational Medicine. Mar, 2015 | Pubmed ID: 25650438 Cardiomyocyte Differentiation of Pluripotent Stem Cells with SB203580 Analogues Correlates with Wnt Pathway CK1 Inhibition Independent of P38 MAPK Signaling Journal of Molecular and Cellular Cardiology. Mar, 2015 | Pubmed ID: 25528965 An Intermittent Rocking Platform for Integrated Expansion and Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes in Suspended Microcarrier Cultures Stem Cell Research. Sep, 2014 | Pubmed ID: 25043964 Cationic Surface Charge Combined with Either Vitronectin or Laminin Dictates the Evolution of Human Embryonic Stem Cells/microcarrier Aggregates and Cell Growth in Agitated Cultures Stem Cells and Development. Jul, 2014 | Pubmed ID: 24641164 Time-resolved Video Analysis and Management System for Monitoring Cardiomyocyte Differentiation Processes and Toxicology Assays Biotechnology Journal. May, 2014 | Pubmed ID: 24619905 Inhibition of ROCK-myosin II Signaling Pathway Enables Culturing of Human Pluripotent Stem Cells on Microcarriers Without Extracellular Matrix Coating Tissue Engineering. Part C, Methods. Mar, 2014 | Pubmed ID: 23777438 Application of Human Mesenchymal and Pluripotent Stem Cell Microcarrier Cultures in Cellular Therapy: Achievements and Future Direction Biotechnology Advances. Nov, 2013 | Pubmed ID: 23531528 Microcarrier Suspension Cultures for High-density Expansion and Differentiation of Human Pluripotent Stem Cells to Neural Progenitor Cells Tissue Engineering. Part C, Methods. Feb, 2013 | Pubmed ID: 22834957 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes on Microcarrier Cultures Current Protocols in Stem Cell Biology. May, 2012 | Pubmed ID: 22605644 Activated T Cells Modulate Immunosuppression by Embryonic-and Bone Marrow-derived Mesenchymal Stromal Cells Through a Feedback Mechanism Cytotherapy. Mar, 2012 | Pubmed ID: 22136295 Translating Human Embryonic Stem Cells from 2-Dimensional to 3-Dimensional Cultures in a Defined Medium on Laminin- and Vitronectin-Coated Surfaces Stem Cells and Development. Dec, 2011 | Pubmed ID: 22034857 Distinct Regulation of Mitogen-activated Protein Kinase Activities is Coupled with Enhanced Cardiac Differentiation of Human Embryonic Stem Cells Stem Cell Research. Nov, 2011 | Pubmed ID: 21907163 Critical Microcarrier Properties Affecting the Expansion of Undifferentiated Human Embryonic Stem Cells Stem Cell Research. Sep, 2011 | Pubmed ID: 21763618 Defining a Threshold Surface Density of Vitronectin for the Stable Expansion of Human Embryonic Stem Cells Tissue Engineering. Part C, Methods. Feb, 2011 | Pubmed ID: 20726687 Agitation Can Induce Differentiation of Human Pluripotent Stem Cells in Microcarrier Cultures Tissue Engineering. Part C, Methods. Feb, 2011 | Pubmed ID: 20698747 Impact of Vitronectin Concentration and Surface Properties on the Stable Propagation of Human Embryonic Stem Cells Biointerphases. Sep, 2010 | Pubmed ID: 21171706 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers Current Protocols in Stem Cell Biology. Sep, 2010 | Pubmed ID: 20814936 Scalable Platform for Human Embryonic Stem Cell Differentiation to Cardiomyocytes in Suspended Microcarrier Cultures Tissue Engineering. Part C, Methods. Dec, 2010 | Pubmed ID: 20590381 Investigations into the Metabolism of Two-dimensional Colony and Suspended Microcarrier Cultures of Human Embryonic Stem Cells in Serum-free Media Stem Cells and Development. Nov, 2010 | Pubmed ID: 20380517 Defined and Serum-free Media Support Undifferentiated Human Embryonic Stem Cell Growth Stem Cells and Development. Jun, 2010 | Pubmed ID: 19686051 KLF4 and PBX1 Directly Regulate NANOG Expression in Human Embryonic Stem Cells Stem Cells (Dayton, Ohio). Sep, 2009 | Pubmed ID: 19522013 Long-term Microcarrier Suspension Cultures of Human Embryonic Stem Cells Stem Cell Research. May, 2009 | Pubmed ID: 19393590 Identification of Cellular Objective for Elucidating the Physiological State of Plasmid-bearing Escherichia Coli Using Genome-scale in Silico Analysis Biotechnology Progress. 2009 | Pubmed ID: 19224555 Quantitative Identification of Teratoma Tissues Formed by Human Embryonic Stem Cells with TeratomEye Biotechnology Letters. May, 2009 | Pubmed ID: 19169887 Generation of High-level Stable Transgene Expressing Human Embryonic Stem Cell Lines Using Chinese Hamster Elongation Factor-1 Alpha Promoter System Stem Cells and Development. Aug, 2008 | Pubmed ID: 18788934 Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow Alan Tin Lun Lam1, Premkumar Jayaraman2, Abigail Becker3, Ryan Lim2, Kim Leng Teo1, Jacqueline Ng2, Steve Oh1 1Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A STAR), 2Thermo Fisher Scientific, 3Thermo Fisher Scientific JoVE 64707 Biochimica
Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow Alan Tin Lun Lam1, Premkumar Jayaraman2, Abigail Becker3, Ryan Lim2, Kim Leng Teo1, Jacqueline Ng2, Steve Oh1 1Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A STAR), 2Thermo Fisher Scientific, 3Thermo Fisher Scientific JoVE 64707 Biochimica