Xianyong Liu Veterinary Parasitology Department China Agricultural University Biography Publications Institution JoVE Articles Xianyong LiuAssociate Professor Xianyong Liu is an Associate Professor in the Veterinary Parasitology Department, College of Veterinary Medicine of China Agricultural University (CAU) in Beijing. He received his undergraduate degree and a Ph.D. from this university.During Dr. Xianyong’s training he optimized the protocol for the development of transient and stable transfection of Eimeria tenella, a protozoa causing cecal coccidiosis in chicken. As a post-doctoral fellow (2012 to 2013) in Prof James Lok’s lab at Pathobiology Department, University of Pennsylvania, US, he tried the TALEN tool in Strongyloides stecoralis.Dr. Xianyong was enrolled as a lecturer at CAU. His research program encompasses eimerian parasites of chicken and rabbits to develop molecular tools for the genetic manipulation of these parasites, as well as to develop vaccines based on the genetically modified eimerian parasites. Publications Immune Protection Provided by a Precocious Line Trivalent Vaccine Against Rabbit Eimeria Veterinary Parasitology. Nov, 2019 | Pubmed ID: 31639571 Selection and Characterization of a Precocious Line of Eimeria Media Parasitology Research. Oct, 2019 | Pubmed ID: 31407118 Co-immunization with Two Recombinant Eimeria Tenella Lines Expressing Immunoprotective Antigens of E. Maxima Elicits Enhanced Protection Against E. Maxima Infection Parasites & Vectors. Jul, 2019 | Pubmed ID: 31300007 Identification of Candidate Antigens by 2-DE Immunoblotting for Diagnosis of Toxoplasma Gondii Infection in Chickens and Rabbits Experimental Parasitology. Sep, 2019 | Pubmed ID: 31299265 Transcriptional Profiling of Innate Immune Responses in Sheep PBMCs Induced by Haemonchus Contortus Soluble Extracts Parasites & Vectors. Apr, 2019 | Pubmed ID: 31023355 Inflammasome Activation Restrains the Intracellular Neospora Caninum Proliferation in Bovine Macrophages Veterinary Parasitology. Apr, 2019 | Pubmed ID: 30981301 Selection and Identification of a Precocious Line of Eimeria Intestinalis with Enlarged Oocysts and Deletion of One Generation of Schizogony Parasitology Research. Mar, 2019 | Pubmed ID: 30694415 Stable Transfection of Eimeria Necatrix Through Nucleofection of Second Generation Merozoites Molecular and Biochemical Parasitology. 03, 2019 | Pubmed ID: 30658178 Comparative Transcriptome Analysis of Eimeria Maxima (Apicomplexa: Eimeriidae) Suggests DNA Replication Activities Correlating with Its Fecundity BMC Genomics. Sep, 2018 | Pubmed ID: 30249186 Eimeria Tenella Infection Perturbs the Chicken Gut Microbiota from the Onset of Oocyst Shedding Veterinary Parasitology. Jul, 2018 | Pubmed ID: 30105975 Transgenic Eimeria Tenella Expressing Profilin of Eimeria Maxima Elicits Enhanced Protective Immunity and Alters Gut Microbiome of Chickens Infection and Immunity. 09, 2018 | Pubmed ID: 29967093 Influence of Eimeria Falciformis Infection on Gut Microbiota and Metabolic Pathways in Mice Infection and Immunity. 05, 2018 | Pubmed ID: 29440368 An Optimized DNA Extraction Method for Molecular Identification of Coccidian Species Parasitology Research. Mar, 2018 | Pubmed ID: 29396674 A Novel Vaccine Delivery Model of the Apicomplexan Expressing Antigen Protects Chickens Against Infection of the Two Parasites Frontiers in Immunology. Month, 2017 | Pubmed ID: 29375584 Improved Cytotoxic T Lymphocyte Responses to Vaccination with Porcine Reproductive and Respiratory Syndrome Virus in 4-1BB Transgenic Pigs Frontiers in Immunology. Month, 2017 | Pubmed ID: 29326720 Increased Neutralizing Antibody Production and Interferon-γ Secretion in Response to Porcine Reproductive and Respiratory Syndrome Virus Immunization in Genetically Modified Pigs Frontiers in Immunology. Month, 2017 | Pubmed ID: 28955336 A New Species of Eimeria (Apicomplexa: Eimeriidae) from Californian Rabbits in Hebei Province, China Parasitology International. Oct, 2017 | Pubmed ID: 28663008 Mucosal IgA and IFN-γ CD8 T Cell Immunity Are Important in the Efficacy of Live Salmonella Enteria Serovar Choleraesuis Vaccines Scientific Reports. 04, 2017 | Pubmed ID: 28406162 Transgenic Pérard, 1925 Displays Similar Parasitological Properties to the Wild-type Strain and Induces an Exogenous Protein-Specific Immune Response in Rabbits ( L.) Frontiers in Immunology. Month, 2017 | Pubmed ID: 28167939 High Pathogenicity and Strong Immunogenicity of a Chinese Isolate of Eimeria Magna Pérard, 1925 Parasitology International. Jun, 2017 | Pubmed ID: 28132801 Calcium-dependent Microneme Protein Discharge and In vitro Egress of Eimeria Tenella Sporozoites Experimental Parasitology. Nov, 2016 | Pubmed ID: 27680997 Transgenic Eimeria Tenella As a Vaccine Vehicle: Expressing TgSAG1 Elicits Protective Immunity Against Toxoplasma Gondii Infections in Chickens and Mice Scientific Reports. 07, 2016 | Pubmed ID: 27387302 Survey of Coccidial Infection of Rabbits in Sichuan Province, Southwest China SpringerPlus. Month, 2016 | Pubmed ID: 27386319 "Self-cleaving" 2A Peptide from Porcine Teschovirus-1 Mediates Cleavage of Dual Fluorescent Proteins in Transgenic Eimeria Tenella Veterinary Research. 06, 2016 | Pubmed ID: 27352927 Chicken IgY Fc Expressed by Eimeria Mitis Enhances the Immunogenicity of E. Mitis Parasites & Vectors. Mar, 2016 | Pubmed ID: 27000834 From the Macro to the Micro: Gel Mapping to Differentiate Between Sporozoites of Two Immunologically Distinct Strains of Eimeria Maxima (Strains M6 and Guelph) PloS One. Month, 2015 | Pubmed ID: 26641262 Immunoproteomic Technology Offers an Extraordinary Diagnostic Approach for Toxoplasma Gondii Infection Journal of Microbiological Methods. Dec, 2015 | Pubmed ID: 26415530 Interferon-Gamma Release Assay: An Effective Tool to Detect Early Toxoplasma Gondii Infection in Mice PloS One. Month, 2015 | Pubmed ID: 26378802 Nitric Oxide Stimulates Early Egress of Toxoplasma Gondii Tachyzoites from Human Foreskin Fibroblast Cells Parasites & Vectors. Aug, 2015 | Pubmed ID: 26264067 Transgenic Eimeria Mitis Expressing Chicken Interleukin 2 Stimulated Higher Cellular Immune Response in Chickens Compared with the Wild-type Parasites Frontiers in Microbiology. Month, 2015 | Pubmed ID: 26082759 Immunogenicity and Protective Efficacy of an Eimeria Vaccine Candidate Based on Eimeria Tenella Immune Mapped Protein 1 and Chicken CD40 Ligand Veterinary Parasitology. May, 2015 | Pubmed ID: 25840621 Exploring Early and Late Toxoplasma Gondii Strain RH Infection by Two-dimensional Immunoblots of Chicken Immunoglobulin G and M Profiles PloS One. Month, 2015 | Pubmed ID: 25803039 Ethanol and Isopropanol Trigger Rapid Egress of Intracellular Eimeria Tenella Sporozoites Parasitology Research. Feb, 2015 | Pubmed ID: 25407127 Protective Immunity Against Eimeria Tenella Infection in Chickens Induced by Immunization with a Recombinant C-terminal Derivative of EtIMP1 Veterinary Immunology and Immunopathology. Dec, 2014 | Pubmed ID: 25464823 Molecular Analysis of Single Oocyst of Eimeria by Whole Genome Amplification (WGA) Based Nested PCR Experimental Parasitology. Sep, 2014 | Pubmed ID: 24996066 CDR3 Analysis of TCR Vβ Repertoire of CD8⁺ T Cells from Chickens Infected with Eimeria Maxima Experimental Parasitology. Aug, 2014 | Pubmed ID: 24801021 An Eimeria Vaccine Candidate Based on Eimeria Tenella Immune Mapped Protein 1 and the TLR-5 Agonist Salmonella Typhimurium FliC Flagellin Biochemical and Biophysical Research Communications. Oct, 2013 | Pubmed ID: 24076159 Interferon-γ Enzyme-linked Immunosorbent Spot Assay As a Tool to Study T Cell Responses to Eimeria Tenella Infection in Chickens Poultry Science. Jul, 2013 | Pubmed ID: 23776262 Molecular Cloning and Protein Characterization of Swine 4-1BB Veterinary Immunology and Immunopathology. May, 2013 | Pubmed ID: 23453329 Expression of Toxoplasma Gondii Dense Granule Protein7 (GRA7) in Eimeria Tenella Parasitology Research. May, 2013 | Pubmed ID: 23371499 Development of Transgenic Lines of Eimeria Tenella Expressing M2e-enhanced Yellow Fluorescent Protein (M2e-EYFP) Veterinary Parasitology. Mar, 2013 | Pubmed ID: 23298569 Plasmid DNA Could Be Delivered into Eimeria Maxima Unsporulated Oocyst with Gene Gun System Acta Veterinaria Hungarica. Dec, 2012 | Pubmed ID: 23160025 PiggyBac Transposon-mediated Transgenesis in the Apicomplexan Parasite Eimeria Tenella PloS One. Month, 2012 | Pubmed ID: 22768223 Molecular Cloning and Characterization of the Full-length CDNA Encoding the Porcine CD28 Veterinary Immunology and Immunopathology. May, 2012 | Pubmed ID: 22510524 Large-scale Survey of the Prevalence of Eimeria Infections in Domestic Rabbits in China Parasitology Research. Apr, 2012 | Pubmed ID: 21972072 High Transfection Efficiency of Porcine Peripheral Blood T Cells Via Nucleofection Veterinary Immunology and Immunopathology. Dec, 2011 | Pubmed ID: 22055481 Transgenic Eimeria Tenella Expressing Enhanced Yellow Fluorescent Protein Targeted to Different Cellular Compartments Stimulated Dichotomic Immune Responses in Chickens Journal of Immunology (Baltimore, Md. : 1950). Oct, 2011 | Pubmed ID: 21876035 Co-expression of Reporter Genes in the Widespread Pathogen Eimeria Tenella Using a Double-cassette Expression Vector Strategy International Journal for Parasitology. Jul, 2011 | Pubmed ID: 21550346 Transfection of Eimeria and Toxoplasma Using Heterologous Regulatory Sequences International Journal for Parasitology. Sep, 2009 | Pubmed ID: 19379753 Dynamic Development of Parasitophorous Vacuole of Eimeria Tenella Transfected with the Yellow Fluorescent Protein Gene Fused to Different Signal Sequences from Apicomplexan Parasites Parasitology Research. Jan, 2009 | Pubmed ID: 18815811 Stable Transfection of Eimeria Tenella: Constitutive Expression of the YFP-YFP Molecule Throughout the Life Cycle International Journal for Parasitology. Jan, 2009 | Pubmed ID: 18718473 Restriction Enzyme-mediated Transfection Improved Transfection Efficiency in Vitro in Apicomplexan Parasite Eimeria Tenella Molecular and Biochemical Parasitology. Sep, 2008 | Pubmed ID: 18606196 Transient Transfection of Eimeria Tenella Using Yellow or Red Fluorescent Protein As a Marker Molecular and Biochemical Parasitology. Jun, 2007 | Pubmed ID: 17399806 Nucleofezione e Propagazione In Vivo dei Parassiti dell'Emeria del Pollo Chunhui Duan1, Xinming Tang1, Dandan Hu1, Sixin Zhang1, Jie Liu1, Feifei Bi1, Zhenkai Hao1, Jingxia Suo1, Ying Yu1, Min Wang1, Pei Sun1, Liying Du2, Xun Suo1, Xianyong Liu1 1National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, 2School of Life Sciences, Peking University JoVE 60552 Biologia
Nucleofezione e Propagazione In Vivo dei Parassiti dell'Emeria del Pollo Chunhui Duan1, Xinming Tang1, Dandan Hu1, Sixin Zhang1, Jie Liu1, Feifei Bi1, Zhenkai Hao1, Jingxia Suo1, Ying Yu1, Min Wang1, Pei Sun1, Liying Du2, Xun Suo1, Xianyong Liu1 1National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, 2School of Life Sciences, Peking University JoVE 60552 Biologia