This video introduces how to perform an oral glucose tolerance test on a laboratory mouse.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Oral Glucose Tolerance Test (OGTT) Glucose solution preparation: Before conducting the experiment, prepare the glucose solution by dissolving 3.75 g glucose in 15 mL distilled H2O. Set up a timetable to record the readings during the experimental procedure (Table 1). …
Representative Results
Table 1. Timetable for Oral Glucose Tolerance Test (OGTT) recording # Mouse ID Body Glucose Start 0 15 30 60 90 weight µL =10xBW time mins</td…
Divulgazioni
The authors have nothing to disclose.
Materials
Glucometer
Roche
#06870244001
Used to measure the blood glucose level
Glucose chip
Roche
#06454011020
Used to load the blood sample
10 mM glucose
Filter sterilize with a 0.22 µm filter apparatus and store at 4°C.