Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity

Évaluation de la différenciation ostéogénique des cellules stromo-endothéliales 3D en surveillant l’activité de la phosphatase alcaline

Published: May 19, 2023

doi:

10.3791/200259

Lisa A. Krattiger, Maria Mitsi, Benjamin R. Simona, Martin Ehrbar

¹Department of Obstetrics,University Hospital Zurich, University of Zurich, ²Ectica Technologies AG

Tags

Keywords: 3D Stromal-endothelial Cells Osteogenic Differentiation Alkaline Phosphatase Activity HBM-MSCs GFP-HUVECs BCIP/NBT Substrate Cell Lysates ALP Activity Quantification DNA Quantification Mesenchymal Stem Cells

Play Video

PDF

DOI

Citazione di questo articolo

Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity. J. Vis. Exp. (Pending Publication), e200259, doi:10.3791/200259 (2023).

Évaluation de la différenciation ostéogénique des cellules stromo-endothéliales 3D en surveillant l’activité de la phosphatase alcaline

Tags

Play Video

Citazione di questo articolo

View Video

✖

To prove you're not a robot, please enter the text in the image below