Orthotopic Homograft Tumor Transplantation: A Technique to Generate Pancreatic Tumor Mouse Models

Abstract

Source: An, X. et al. Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry. J. Vis. Exp. (2018)

The video explains a stepwise surgical method for orthotopic implantation of a tumor fragment from a cancerous donor to the pancreas of a non-cancerous recipient mice for development of pancreatic cancer. Orthotopically implanted PDAC could recapture the tissue microenvironment of human pancreatic cancers than conventional subcutaneous (SC) models more accurately.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation for Tumor Transplantation Animal Housing Obtain generic C57BL/6 mice from a commercial breeding vendor. House mice (5) in individual ventilated cages at the following conditions: Temperature: 20–26 °C; humidity 30–70%; lighting cycle: 12 h light and 12…

Materials

Iodophor swabs			Daily pharmacy purchase
Alcohol swabs			Daily pharmacy purchase
PBS	Hyclone	SH30256.01	50 mL
Disposable, sterile scalpels	Jin zhong	J12100	11#
Studylog-Data line of vernier calipers	Sylvac	926.6721	Data line of vernier calipers
Caliper	Sylvac	910.1502.10	Sylvac S-Cal pro
eye scissors and tweezers	Jin zhong	Y00030	Eye scissors 10 cm
Tumor Dissociation Kit	Miltenyi	130-096-730
eye scissors and tweezers	Jin zhong	JD1060	Eye tweezers 10 cm with teeth
eye scissors and tweezers		SAS3119
eye scissors and tweezers	Hyclone	SH30809.01
eye scissors and tweezers	Thermo	KLCS-288	4 °C
eye scissors and tweezers	Metter Toledo	AL204 0-100 g
100x antibiotic and antimycotic
Anesthesia machine		SAS3119
Ice bucket	Thermo	KLCS-288	4 °C