Mouse Antral Oocyte Isolation: A Method to Isolate Antral Oocytes from Freshly Harvested Mouse Ovaries

Abstract

Source: Monti, M. et al. Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization. J. Vis. Exp. (2016)

This video describes the isolation of antral oocytes from freshly harvested mouse ovaries. These antral oocytes can be used for further characterization and developmental studies.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Animals Maintain adult 3- to 6-week-old female mice in a room with controlled temperature and humidity (with phases of 12L:12D and fed ad libitum). Inject mice intraperitoneally with 2.5 IU of Pregnant mare serum gonadotropin (PMSG) to stimulate follicular growth by mimicking the effects of folli…

Representative Results

Figure 1. Preparation of a mouth pipette using glass Pasteur pipettes and a Bunsen burner. Figure 2. Intact ovaries in Petri dish filled with pre-warmed and equilibrated M2 medium.</stron…

Materials

PMSG	Sigma	G4527
EmbrioMax M2	Millipore	MR-015-PD
Mineral Oil	Sigma	M8410
Cell Culture Dish 35 mm x 10 mm	Corning	430165
µ-Dish 35 mm, high glass bottom	Ibidi	81158
Pipette Pasteur Borosilicate	Corning	7095D-9
Aspirator tube assemblies for calibrated micropillary pipettes	Sigma	A5177